来自上伊瓜苏河（巴拉那州）的阿氏脂鲤属D种（脂鲤目：脂鲤科）组成型异染色质的特征分析

Characterization of the constitutive heterochromatin of Astyanax sp. D (Characiformes: Characidae) from the Upper Iguaçu River (PR).

作者信息

Kantek Daniel Luis Zanella, Cestari Marta Margarete, Bertollo Luiz Antônio Carlos, Moreira-Filho Orlando

机构信息

Laboratório de Biodiversidade Molecular e Citogenética, Universidade Federal de São Carlos, CEP 13566-430, São Carlos, SP, Brazil.

出版信息

Folia Biol (Krakow). 2009;57(1-2):37-41. doi: 10.3409/fb57_1-2.37-41.

DOI:10.3409/fb57_1-2.37-41

PMID:19459459

Abstract

Chromosomal characterization of constitutive heterochromatin was performed in Astyanax sp. D, a species found in the Iguaçu river, using C-banding, fluorescent in situ hybridization (FISH) of the repetitive DNA family As51, localization of the 18S rDNA sites and base-specific fluorochromes. The sites of the satellite DNA coincide with large heterochromatic blocks present in this species, namely CMA3- and DAPI-. The many 18S rDNA sites are CMA3+. Similarity between the large heterochromatic blocks is suggested, as well as the identification of two types of heterochromatin, one related to the 18S rDNA domains and the other to the As51 regions.

摘要

利用C带、重复DNA家族As51的荧光原位杂交（FISH）、18S rDNA位点定位和碱基特异性荧光染料，对在伊瓜苏河发现的一种阿氏丽脂鲤（Astyanax sp. D）的组成型异染色质进行了染色体特征分析。卫星DNA的位点与该物种中存在的大型异染色质块一致，即CMA3阳性和DAPI阴性。许多18S rDNA位点是CMA3阳性。结果表明大型异染色质块之间存在相似性，同时鉴定出两种类型的异染色质，一种与18S rDNA结构域相关，另一种与As51区域相关。