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关于将1978年卡蒂和利奇菲尔德鉴定的海洋沉积物黄杆菌转移至短小杆菌属,命名为海洋沉积物短小杆菌(新组合)的提议

Proposal to transfer Flavobacterium oceanosedimentum Carty and Litchfield 1978 to the genus Curtobacterium as Curtobacterium oceanosedimentum comb. nov.

作者信息

Kim Byung Kwon, Kim Ok-Sun, Moon Eun Young, Chun Jongsik

机构信息

School of Biological Sciences, Seoul National University, Seoul, Korea.

出版信息

FEMS Microbiol Lett. 2009 Jul;296(1):137-41. doi: 10.1111/j.1574-6968.2009.01628.x. Epub 2009 May 5.

Abstract

Flavobacterium oceanosedimentum Carty & Litchfield 1978(AL) had been described as a novel species in the genus Flavobacterium on the basis of phenotypic characteristics, although the organism shows a high G+C content, a property never found in the other members of the genus. In this study, we re-evaluated the taxonomic position of F. oceanosedimentum using a polyphasic approach. The 16S rRNA gene sequence of F. oceanosedimentum ATCC 31717(T) indicated that it is closely related to the genus Curtobacterium in the class Actinobacteria, showing 96.5-99.9% similarity values to Curtobacterium spp. Phylogenetic analysis based on the gene encoding the gyrase subunit B (gyrB) and DNA-DNA hybridization suggest that F. oceanosedimentum represents a separate species in the genus Curtobacterium, which is also supported by comparative analysis of cellular fatty acid profiles and a large array of phenotypic traits. We therefore propose to transfer F. oceanosedimentum to the genus Curtobacterium as Curtobacterium oceanosedimentum comb. nov.

摘要

海洋沉积物黄杆菌(Flavobacterium oceanosedimentum)Carty和Litchfield于1978年(AL)基于表型特征被描述为黄杆菌属中的一个新物种,尽管该菌显示出高G+C含量,这是该属其他成员从未发现的特性。在本研究中,我们采用多相分类方法重新评估了海洋沉积物黄杆菌的分类地位。海洋沉积物黄杆菌ATCC 31717(T)的16S rRNA基因序列表明,它与放线菌纲中的短小杆菌属密切相关,与短小杆菌属各菌株的相似性值为96.5 - 99.9%。基于编码gyrase亚基B(gyrB)的基因和DNA-DNA杂交的系统发育分析表明,海洋沉积物黄杆菌代表短小杆菌属中的一个独立物种,细胞脂肪酸谱和大量表型特征的比较分析也支持这一点。因此,我们建议将海洋沉积物黄杆菌转移到短小杆菌属,命名为海洋沉积物短小杆菌(Curtobacterium oceanosedimentum)新组合。

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