Stackebrandt Erko, Päuker Orsola, Steiner Ulrike, Schumann Peter, Sträubler Bettina, Heibei Sandra, Lang Elke
DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Mascheroder Weg 1b, 38124 Braunschweig, Germany.
Syst Appl Microbiol. 2007 Mar;30(2):109-18. doi: 10.1016/j.syapm.2006.03.002. Epub 2006 Apr 3.
Corallococcus coralloides DSM 2259(T), Corallococcus exiguus DSM 14696(T), Corallococcus macrosporus DSM 14697(T) and more than 35 strains identified as members of Corallococcus on the basis of morphology were subjected to partial sequences analysis of three housekeeping genes (lepA, fusA and rpoB), complementing a recent phylogenetic analysis based on genes coding for 16S rRNA and gyrB. Phylogenetic analysis of each gene, generated by maximum likelihood and two different additive treeing algorithms, resulted in the separate position of C. macrosporus DSM 14697(T) and a few Corallococcus strains that were more closely related to Myxococcus xanthus than to the other members of Corallococcus. The latter strains formed three clearly separate clusters by 16S rRNA gene phylogeny. This relationship, however, was only partially recovered by the other gene trees. Group 1, embracing the type strains of C. coralloides and C. exiguus, only emerged as a coherent cluster in the 16S rRNA gene tree. In all other gene trees this cluster embraced organisms of cluster 3, which either formed coherent subclusters (gyrB, lepA) or which appeared polyphyletic (fusA, rpoB). Group 2 organisms consistently constituted a monophyletic cluster, though their branching within the gene trees differed. A concatenated tree, based on the analysis of about 5400 nucleotides of all five partial genes was most similar to the 16S rRNA gene tree. In order to determine whether the individual clusters that emerged by 16S rRNA gene analysis (>99.1% intracluster similarities) show phenetic properties that would allow their description as new species, a few strains of each group were subjected to the analysis of whole cell fatty acid and physiological properties. Riboprint patterns were generated for some members of group 1. While the DNA-DNA reassociation values and riboprint patterns confirmed the genomic heterogeneity of members of cluster 1, none of the other properties investigated were sufficiently discriminative to allow the formal description of strain clusters as new species.
对珊瑚球菌DSM 2259(T)、微小珊瑚球菌DSM 14696(T)、大孢珊瑚球菌DSM 14697(T)以及35株以上基于形态学鉴定为珊瑚球菌属成员的菌株进行了三个管家基因（lepA、fusA和rpoB）的部分序列分析，作为对最近基于16S rRNA和gyrB编码基因的系统发育分析的补充。通过最大似然法和两种不同的相加树算法对每个基因进行系统发育分析，结果显示大孢珊瑚球菌DSM 14697(T)以及一些与黄色粘球菌的亲缘关系比与其他珊瑚球菌属成员更近的珊瑚球菌菌株处于单独的位置。后一类菌株通过16S rRNA基因系统发育形成了三个明显分开的簇。然而，这种关系在其他基因树中仅部分恢复。第1组包括珊瑚球菌和微小珊瑚球菌的模式菌株，仅在16S rRNA基因树中作为一个连贯的簇出现。在所有其他基因树中，这个簇包含第3簇的生物体，它们要么形成连贯的亚簇（gyrB、lepA），要么表现为多系发生（fusA、rpoB）。第2组生物体始终构成一个单系簇，尽管它们在基因树中的分支不同。基于对所有五个部分基因约5400个核苷酸的分析构建的串联树与16S rRNA基因树最为相似。为了确定通过16S rRNA基因分析出现的各个簇（簇内相似性>99.1%）是否表现出足以将其描述为新物种的表型特征，对每组的一些菌株进行了全细胞脂肪酸和生理特性分析。为第1组中的一些成员生成了核糖体印记图谱。虽然DNA-DNA重结合值和核糖体印记图谱证实了第1簇成员的基因组异质性，但所研究的其他特性均没有足够的鉴别力来允许将菌株簇正式描述为新物种。
