[靶向CXCR4的小干扰RNA对乳腺癌血管生成的影响]
[Effect of small interfering RNA targeting CXCR4 on breast cancer angiogenesis].
作者信息
Li Hong-yuan, Ren Guo-sheng, Tan Jin-xiang
机构信息
Department of General Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2009 May;29(5):954-8.
OBJECTIVE
To study the effects of a small interfering RNA targeting CXCR-4 (shRNA-CXCR4) on angiogenesis of human breast cancer cells.
METHODS
The expression of CXCR4 mRNA and protein in 3 breast cancer cell lines with CXCR-4 silencing mediated by shRNA-CXCR4 was detected by RT-PCR and Western blotting, respectively. The morphological changes of human umbilical vein endothelial cells (HUVECs) were observed in co-culture with human breast cancer cells after CXCR4 gene silencing.
RESULTS
CXCR4 mRNA and protein expressions decreased significantly in MCF-7, MDA-MB-231 and MDA-MB-435s breast cancer cells after the gene silencing (P<0.05). Gene silencing with shRNA-CXCR4 in human breast cancer cells significantly inhibited the ability of HUVECs to form tubular structures in the co-culture (P<0.05).
CONCLUSION
Gene silencing by shRNA-CXCR4 can obviously lower the angiogenesis-inducing ability of human breast cancer cells.
目的
研究靶向趋化因子受体4（CXCR-4）的小干扰RNA（shRNA-CXCR4）对人乳腺癌细胞血管生成的影响。
方法
分别采用逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测经shRNA-CXCR4介导CXCR-4沉默的3种乳腺癌细胞系中CXCR4 mRNA和蛋白的表达。观察CXCR4基因沉默后人乳腺癌细胞与脐静脉内皮细胞（HUVECs）共培养时HUVECs的形态变化。
结果
基因沉默后，MCF-7、MDA-MB-231和MDA-MB-435s乳腺癌细胞中CXCR4 mRNA和蛋白表达显著降低（P<0.05）。shRNA-CXCR4对人乳腺癌细胞进行基因沉默可显著抑制共培养体系中HUVECs形成管状结构的能力（P<0.05）。
结论
shRNA-CXCR4介导的基因沉默可明显降低人乳腺癌细胞的血管生成诱导能力。
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