[shRNA-CXCR4对乳腺癌细胞迁移和侵袭的影响]
[The effects of shRNA-CXCR4 on breast cancer cells migration and invasion].
作者信息
Li Hong-yuan, Ren Guo-sheng, Tan Jin-xiang
机构信息
The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 May;40(3):393-7.
OBJECTIVE
To study the effect of shRNA-CXCR4 on human breast cancer cells migration and invasion.
METHODS
Through knockdown CXCR4 by shRNA, the CXCR4 mRNA expression and protein expression were examined with RT-PCR and Western blot separately. The matrigel penetrating ability of human breast cancer cells was examined in vitro experiments.
RESULTS
The CXCR4 mRNA express and its protein express decreased significantly in MCF-7, MDA-MB-231 and MDA-MB-435s breast cancer cells (P<0.05). The invasive ability of human breast cancer cells was significantly decreased (P<0.05).
CONCLUSION
through knockdown CXCR4 by shRNA, the invasive ability of human breast cancer cells can be inhibited obviously.
目的
研究shRNA-CXCR4对人乳腺癌细胞迁移和侵袭的影响。
方法
通过shRNA敲低CXCR4，分别采用RT-PCR和蛋白质免疫印迹法检测CXCR4 mRNA表达和蛋白表达。在体外实验中检测人乳腺癌细胞的基质胶穿透能力。
结果
MCF-7、MDA-MB-231和MDA-MB-435s乳腺癌细胞中CXCR4 mRNA表达及其蛋白表达显著降低（P<0.05）。人乳腺癌细胞的侵袭能力显著降低（P<0.05）。
结论
通过shRNA敲低CXCR4，可明显抑制人乳腺癌细胞的侵袭能力。
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