Fuma Shoichi, Nakamori Taizo, Ishii Nobuyoshi, Kubota Yoshihisa, Yoshida Satoshi, Fujimori Akira
Environmental Radiation Effects Research Group, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba, 263-8555, Japan.
Bull Environ Contam Toxicol. 2009 Sep;83(3):301-6. doi: 10.1007/s00128-009-9780-y. Epub 2009 May 22.
Transcriptome was analyzed in gamma-irradiated green alga Pseudokirchneriella subcapitata by high coverage gene expression profiling (HiCEP). Approximately 7,800 expressed genes were detected. Expression levels of 623-707 genes were affected at 100-300 Gy. Nucleotide sequences of 41 up-regulated genes were determined. The quantitative reverse transcription polymerase chain reaction validated the up-regulation. Two genes had homology to genes related to ionizing radiation. These results indicate usefulness of HiCEP for screening of stress-responsive genes in species that are ecotoxicologically important but for which genomic sequence information is lacking.
