Zhao Ju-mei, Liu Tao, Wei Yu-quan, Wen Yan-jun, Wu Yang
Department of Pharmacology, Medical College of Yan'an University, Yan'an 716000, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Mar;40(2):208-11.
To test the inhibitive effect of the matrix protein of vesicular stomatitis virus (VSV) on the proliferation of colon carcinoma cells of mice in vitro.
The plasmid pcDNA3. 1-M encoding M protein of vesicular stomatitis viruses was transfected with lipofectamine 2000 into the colon carcinoma (CT26) cells of mice. The expression of VSV-M protein in the CT26 cells was detected by Western blot. The effect of vesicular stomatitis virus (VSV) matrix protein on the proliferation and apoptosis of colon carcinoma cells was measured by MTT, PI stainning and flow cytometry.
The plasmid expressed M protein in the CT26 cells. Cytopathic effect was shown in the CT26 cells with transfected pcDNA3.1-M. The proliferation of the CT26 cells was suppressed significantly in vitro (76.4%, P < 0.05). The M protein induced apoptosis of the CT26 cells (80.2%), which was higher than'that of the controls (P < 0.05).
The eukaryotic expression plasmid pcDNA3.1-M encoding M protein of vesicular stomatitis viruses induces apoptosis of CT26 cells, which has implications on the treatment of human colon cancer.
检测水疱性口炎病毒（VSV）基质蛋白对小鼠结肠癌细胞体外增殖的抑制作用。
采用脂质体2000将编码水疱性口炎病毒M蛋白的质粒pcDNA3.1-M转染至小鼠结肠癌（CT26）细胞。通过蛋白质免疫印迹法检测CT26细胞中VSV-M蛋白的表达。采用MTT法、碘化丙啶（PI）染色法及流式细胞术检测水疱性口炎病毒（VSV）基质蛋白对结肠癌细胞增殖和凋亡的影响。
该质粒在CT26细胞中表达M蛋白。转染pcDNA3.1-M的CT26细胞出现细胞病变效应。体外实验中，CT26细胞的增殖受到显著抑制（76.4%，P<0.05）。M蛋白诱导CT26细胞凋亡（80.2%），高于对照组（P<0.05）。
编码水疱性口炎病毒M蛋白的真核表达质粒pcDNA3.1-M可诱导CT26细胞凋亡，这对人类结肠癌的治疗具有重要意义。
