[抗黏液病毒1和2',5'-寡腺苷酸合成酶1与系统性红斑狼疮临床特征的相关性]

[The correlation of myxovirus resistance 1 and 2'5'-ollgoadenylate synthetase 1 with clinical features of systemic lupus erythematosus].

作者信息

Li Min, Yang Nan-ping, Huang Xiang-yang, Zhao Zhen-yi, Zhang Jie, Liu Xiao-xiao, Pu Xiao-ying, Dong Jian-ling, Ye Yang

机构信息

Department of Rheumatology, West China Hospital, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Mar;40(2):268-71.

PMID:19462904

Abstract

OBJECTIVE

To investigate the expression of myxovirus resistance 1(MX1) and 2'5'-oligoadenylate synthetase 1(OAS1) genes in the peripheral blood leukocyte of patients with systemic lupus erythematosus (SLE), and to evaluated the relations between these genes expression levels and disease activity.

METHODS

In this study, there were 50 SLE petients, 20 non-SLE patients with rheumatic dieases, and 25 normal controls. The peripheral blood samples of these patients were collected, and the expression levels (indicated as delta Ct value) of MX1 and OAS1 were measured by Sybr green dye based real-time quantitative PCR method.

RESULTS

The deltaCt value of MX1 expression in SLE patients was 3.55 +/- 1.39, which was significantly higher than those of non-SLE patients (2.31 +/- 0.52, P = 0.000) and normal controls (2.23 +/- 1.05, P = 0.000). (2) The deltaCt value of OAS1 expression in SLE patients was 4.45 +/- 1.56, which also was significantly higher than those of non-SLE patients (3.03 +/- 0.76, P = 0.000) and normal controls (2.75 +/- 0.64, P = 0.000). (3) The deltaCt value of OAS1 was correlated with the SLEDAI scores (r = 0.338, P = 0.019) and serum IgA level. (4) The ACt value of MX1 was not correlated with the SLEDAI scores (r = 0.064, P = 0.661), but correlated with the serum levels of triglyceride (TG), cholesterol (TC), low density lipoprotein (LDL), albuminuria of 24 hours (r = 0.428, P = 0.003 r = 0.383, P = 0.009 r = 0.394, P = 0.007; r = 0.316, P = 0.025); (5) The deltaCt values of MX1 and OAS1 in the SLE patients with arthritis were significantly higher than those in non-arthritis SLE patients (3.04 +/- 1.42, P = 0.004; 3.89 +/- 1.49, P = 0.006).

CONCLUSION

The expression levels of both MX1 and OAS1 in SLE patients are up-regulated, the expression levels of OAS1 genes are associated with SLE disease activity. As IFN-induced genes, MX1 and OAS1 play their respective role in SLE.

摘要

目的

研究黏液病毒抗性蛋白1（MX1）和2′,5′-寡腺苷酸合成酶1（OAS1）基因在系统性红斑狼疮（SLE）患者外周血白细胞中的表达情况，并评估这些基因表达水平与疾病活动度之间的关系。

方法

本研究纳入50例SLE患者、20例非SLE风湿性疾病患者和25例正常对照。采集这些患者的外周血样本，采用基于SYBR Green染料的实时定量PCR法检测MX1和OAS1的表达水平（以ΔCt值表示）。

结果

（1）SLE患者MX1表达的ΔCt值为3.55±1.39，显著高于非SLE患者（2.31±0.52，P=0.000）和正常对照（2.23±1.05，P=0.000）。（2）SLE患者OAS1表达的ΔCt值为4.45±1.56，也显著高于非SLE患者（3.03±0.76，P=0.000）和正常对照（2.75±0.64，P=0.000）。（3）OAS1的ΔCt值与SLE疾病活动指数（SLEDAI）评分相关（r=0.338，P=0.019），并与血清IgA水平相关。（4）MX1的ΔCt值与SLEDAI评分无关（r=0.064，P=0.661），但与甘油三酯（TG）、胆固醇（TC）、低密度脂蛋白（LDL）水平以及24小时蛋白尿相关（r=0.428，P=0.003；r=0.383，P=0.009；r=0.394，P=0.007；r=0.316，P=0.025）；（5）有关节炎的SLE患者中MX1和OAS1的ΔCt值显著高于无关节炎的SLE患者（3.04±1.42，P=0.004；3.89±1.49，P=0.006）。