Perepelov A V, Liu B, Senchenkova S N, Shashkov A S, Feng L, Wang L, Knirel Y A
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia.
Biochemistry (Mosc). 2009 Apr;74(4):416-20. doi: 10.1134/s0006297909040099.
An O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Salmonella enterica O47 and studied by sugar analysis along with one- and two-dimensional 1H- and 13C-NMR spectroscopy. The following structure of the linear ribitol phosphate-containing repeating unit of the O-polysaccharide was established: -->2)-D-Ribitol-5-P-(O-->6)-alpha-D-Galp-(1-->3)-alpha-L-FucpNAm-(1-->3)-beta-D-GlcpNAc-(1-->, where FucNAm stands for 2-acetimidoylamino-2,6-dideoxy-L-galactose. About 10% of Gal is O-acetylated at position 4 and another minor O-acetyl group is present at an undetermined position. Functions of the S. enterica O47 antigen biosynthetic genes were tentatively assigned by comparison with gene databases and found to be in agreement with the O-polysaccharide structure. A comparison of the O-antigen gene clusters of S. enterica O47 and E. coli O145 suggested their close evolutionary relationship.
通过温和酸降解肠炎沙门氏菌O47的脂多糖分离得到一种O-多糖，并通过糖分析以及一维和二维1H-和13C-NMR光谱对其进行了研究。确定了O-多糖含线性磷酸核糖醇的重复单元的以下结构：→2)-D-核糖醇-5-P-(O→6)-α-D-吡喃半乳糖-(1→3)-α-L-岩藻糖胺-(1→3)-β-D-吡喃葡萄糖胺-(1→，其中FucNAm代表2-乙酰亚氨基氨基-2,6-二脱氧-L-半乳糖。约10%的半乳糖在4位被O-乙酰化，另一个较小的O-乙酰基存在于未确定的位置。通过与基因数据库比较，初步确定了肠炎沙门氏菌O47抗原生物合成基因的功能，发现与O-多糖结构一致。肠炎沙门氏菌O47和大肠杆菌O145的O抗原基因簇比较表明它们具有密切的进化关系。
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