Yan Feng, Wu Jie, Tan Fang, Yan Yuetian, Ju Huangxian
Jiangsu Institute of Cancer Prevention and Cure, Nanjing 210093, PR China.
Anal Chim Acta. 2009 Jun 30;644(1-2):36-41. doi: 10.1016/j.aca.2009.04.017. Epub 2009 Apr 18.
A sensitive, rapid and simple method for analysis of protein was proposed by introducing an electric field-driven technique to the incubation process of immunoassay and immobilizing horseradish peroxidase (HRP) labeled antibody in a newly designed gel matrix to construct an immunosensor. With an electric field-driven technique, the incubation for immuno-recognition could be completed within 2 min. The immobilized HRP showed excellent direct electrochemistry in this new gel matrix, and the detection procedure was greatly simplified by directly monitoring the sensitive electrochemical signal of HRP upon the immunoreaction. Using alpha-fetoprotein as a model the linear detection range was from 0.02 to 2.0 ng mL(-1) with a detection limit down to 138 amol mL(-1). The immunosensor and the proposed detection method showed good specificity and acceptable stability and accuracy.
通过将电场驱动技术引入免疫分析的孵育过程，并将辣根过氧化物酶（HRP）标记的抗体固定在新设计的凝胶基质中构建免疫传感器，提出了一种灵敏、快速且简单的蛋白质分析方法。利用电场驱动技术，免疫识别的孵育可在2分钟内完成。固定化的HRP在这种新型凝胶基质中表现出优异的直接电化学性能，通过在免疫反应时直接监测HRP的灵敏电化学信号，大大简化了检测程序。以甲胎蛋白为模型，线性检测范围为0.02至2.0 ng mL(-1)，检测限低至138 amol mL(-1)。该免疫传感器及所提出的检测方法具有良好的特异性、可接受的稳定性和准确性。
