无载体的HIV合成肽在体内引发抗人类免疫缺陷病毒（HIV）的CD8 + 细胞毒性T细胞。
Priming of anti-human immunodeficiency virus (HIV) CD8+ cytotoxic T cells in vivo by carrier-free HIV synthetic peptides.
作者信息
Hart M K, Weinhold K J, Scearce R M, Washburn E M, Clark C A, Palker T J, Haynes B F
机构信息
Department of Medicine, Duke University Medical Center, Durham, NC 27710.
出版信息
Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9448-52. doi: 10.1073/pnas.88.21.9448.
Abstract
The generation of antiviral cytotoxic T lymphocytes (CTLs) is a critical component of the immune response to viral infections. A safe and nontoxic vaccine for AIDS would optimally use a carrier-free synthetic peptide immunogen containing only components of HIV necessary for induction of protective immune responses. We report that hybrid synthetic peptides containing either a HIV envelope gp120 T-cell determinant (T1) or the envelope gp41 fusion domain (F) N-terminal to HIV CTL determinants are capable of priming murine CD8+, major histocompatibility complex class I-restricted anti-HIV CTLs in vivo. These data demonstrate that carrier-free, nonderivatized synthetic peptides can be used in vivo to induce anti-HIV CTL responses.
摘要
抗病毒细胞毒性T淋巴细胞（CTL）的产生是针对病毒感染的免疫反应的关键组成部分。一种安全无毒的艾滋病疫苗将理想地使用一种无载体的合成肽免疫原，该免疫原仅包含诱导保护性免疫反应所需的HIV成分。我们报告，含有HIV包膜糖蛋白120 T细胞决定簇（T1）或HIV CTL决定簇N端的包膜糖蛋白41融合域（F）的杂合合成肽能够在体内启动小鼠CD8 +、主要组织相容性复合体I类限制的抗HIV CTL。这些数据表明，无载体、未衍生化的合成肽可在体内用于诱导抗HIV CTL反应。
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