Andreone Luz, Velásquez Ethel V, Abramovich Dalhia, Ambao Verónica, Loreti Nazareth, Croxatto Horacio B, Parborell Fernanda, Tesone Marta, Campo Stella
Centro de Investigaciones Endocrinológicas (CEDIE-CONICET), Hospital de Niños R. Gutiérrez, Gallo 1330, C1425EFD, Buenos Aires, Argentina.
Mol Cell Endocrinol. 2009 Oct 15;309(1-2):48-54. doi: 10.1016/j.mce.2009.05.003. Epub 2009 May 21.
The aim of the present study was to determine the endocrine activity of cultured early antral follicles (EAF) isolated from prepubertal diethylstilbestrol-treated rats. The effect of steroidogenic substrates and FSH on steroid, inhibin A and B, Pro-alphaC and activin A production was evaluated. Androsterone was the predominant steroid produced by EAF. The addition of androstenedione, androstenedione+FSH and progesterone stimulated oestradiol production, whereas 25-hydroxycholesterol (25-OH-Chol) increased progesterone production. Inhibin A, B, Pro-alphaC, and activin A were produced under basal conditions. The predominance of inhibin B over inhibin A was not affected by the addition of androstenedione or progesterone. Inhibin A and activin A production was stimulated by FSH. 25-OH-Chol increased Inha, Inhba and Inhbb mRNA expression and the production of the three molecular forms of inhibins but decreased activin A production. These results show that FSH and the steroid follicular microenvironment differentially modulate the gene expression of inhibin/activin subunits, their assembly and secretion.
本研究的目的是确定从青春期前己烯雌酚处理大鼠分离的培养早期窦卵泡（EAF）的内分泌活性。评估了类固醇生成底物和促卵泡激素（FSH）对类固醇、抑制素A和B、前αC和激活素A产生的影响。雄酮是EAF产生的主要类固醇。添加雄烯二酮、雄烯二酮+FSH以及孕酮可刺激雌二醇的产生，而25-羟基胆固醇（25-OH-Chol）可增加孕酮的产生。在基础条件下可产生抑制素A、B、前αC和激活素A。抑制素B相对于抑制素A的优势不受添加雄烯二酮或孕酮的影响。FSH可刺激抑制素A和激活素A的产生。25-OH-Chol增加了抑制素α（Inha）、抑制素βA（Inhba）和抑制素βB（Inhbb）mRNA的表达以及三种分子形式抑制素的产生，但减少了激活素A的产生。这些结果表明，FSH和类固醇卵泡微环境对抑制素/激活素亚基的基因表达、它们的组装和分泌有不同的调节作用。
