Pokorná Dana, Poláková Ingrid, Kindlová Martina, Dusková Martina, Ludvíková Viera, Gabriel Pavel, Kutinová Lud'a, Müller Martin, Smahel Michal
Institute of Hematology and Blood Transfusion, Department of Experimental Virology, U Nemocnice 1, 12820 Prague 2, Czech Republic.
Vaccine. 2009 Jun 2;27(27):3519-29. doi: 10.1016/j.vaccine.2009.03.073. Epub 2009 Apr 16.
Tattooing has been shown to be very efficient at inducing immunity by vaccination with DNA vaccines. In this study, we examined the usability of tattooing for delivery of peptide vaccines. We compared tattooing with subcutaneous (s.c.) needle injection using peptides derived from human papillomavirus type 16 (HPV16) proteins. We observed that higher peptide-specific immune responses were elicited after vaccination with the simple peptides (E7(44-62) and E7(49-57)) and keyhole limpet hemocyanin-(KLH)-conjugated peptides (E7(49-57), L2(18-38) and L2(108-120)) with a tattoo device compared to s.c. inoculation. The administration of the synthetic oligonucleotide containing immunostimulatory CpG motifs (ODN1826) enhanced the immune responses developed after s.c. injection of some peptides (E7(44-62), KLH-conjugated L2(18-38) and L2(108-120)) to levels close to or even comparable to those after tattoo delivery of identical peptides with ODN1826. The highest efficacy of tattooing was observed in combination with ODN1826 for the vaccination with the less immunogenic E6(48-57) peptide and KLH-conjugated and non-conjugated E7(49-57) peptides which form the visible aggregates that could negatively influence the development of immune responses after s.c. injection but probably not after tattooing. In summary, we first evidenced that tattoo administration of peptide vaccines that might be useful in some cases efficiently induced both humoral and cell-mediated immune responses.
纹身已被证明在通过DNA疫苗接种诱导免疫方面非常有效。在本研究中，我们研究了纹身用于递送肽疫苗的可行性。我们使用源自人乳头瘤病毒16型（HPV16）蛋白的肽，将纹身与皮下（s.c.）针头注射进行了比较。我们观察到，与皮下接种相比，使用纹身装置接种简单肽（E7(44 - 62)和E7(49 - 57)）以及钥孔戚血蓝蛋白（KLH）偶联肽（E7(49 - 57)、L2(18 - 38)和L2(108 - 120)）后，能引发更高的肽特异性免疫反应。含有免疫刺激CpG基序的合成寡核苷酸（ODN1826）的给药增强了皮下注射某些肽（E7(44 - 62)、KLH偶联的L2(18 - 38)和L2(108 - 120)）后产生的免疫反应，使其水平接近甚至与用ODN1826纹身递送相同肽后的水平相当。在与ODN1826联合用于接种免疫原性较低的E6(48 - 57)肽以及KLH偶联和未偶联的E7(49 - 57)肽时，观察到纹身的最高效力，这些肽形成可见聚集体，可能会对皮下注射后的免疫反应发展产生负面影响，但对纹身接种可能没有影响。总之，我们首次证明，在某些情况下可能有用的肽疫苗纹身接种能有效诱导体液免疫和细胞介导的免疫反应。
