广宿主范围IncQ质粒的复制与接合转移
Replication and conjugative mobilization of broad host-range IncQ plasmids.
作者信息
Meyer Richard
机构信息
Institute for Cell and Molecular Biology, University of Texas at Austin, 78712-0162, USA.
出版信息
Plasmid. 2009 Sep;62(2):57-70. doi: 10.1016/j.plasmid.2009.05.001. Epub 2009 May 22.
Abstract
The IncQ plasmids have a broader host-range than any other known replicating element in bacteria. Studies on the replication and conjugative mobilization of these plasmids, which have mostly been focused on the nearly identical RSF1010 and R1162, are summarized with a view to understanding how this broad host-range is achieved. Several significant features of IncQ plasmids emerge from these studies: (1) initiation of replication, involving DnaA-independent activation of the origin and a dedicated primase, is strictly host-independent. (2) The plasmids can be conjugatively mobilized by a variety of different type IV transporters, including those engaged in the secretion of proteins involved in pathogenesis. (3) Stability is insured by a combination of high copy-number and modulated gene expression to reduce metabolic load.
摘要
IncQ质粒比细菌中任何其他已知的复制元件都具有更广泛的宿主范围。对这些质粒的复制和接合转移的研究主要集中在几乎相同的RSF1010和R1162上，为了理解这种广泛的宿主范围是如何实现的，现将这些研究进行总结。这些研究揭示了IncQ质粒的几个重要特征：（1）复制起始涉及不依赖DnaA对复制起点的激活以及一种特定的引发酶，严格不依赖宿主。（2）这些质粒可以被多种不同的IV型转运蛋白进行接合转移，包括那些参与致病相关蛋白分泌的转运蛋白。（3）通过高拷贝数和调节基因表达以降低代谢负担相结合来确保稳定性。
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