L'Homme Yvan, Sansregret Raphaël, Simard Carole
Canadian Food Inspection Agency, St-Hyacinthe Laboratory, 3400 Blvd Casavant West, St-Hyacinthe, Quebec J2S 8E3, Canada. yvan.l'
Food Microbiol. 2009 Aug;26(5):552-5. doi: 10.1016/j.fm.2009.03.011. Epub 2009 Apr 5.
Conventional broad range RT-PCR and real time RT-PCR assays designed to detect human noroviruses (NoVs) also efficiently detect swine NoVs. Investigation of the primers and probe binding sites revealed strong homologies between swine NoVs genomic sequences and human primer sequences. These findings have a serious impact on food diagnostic methods and laboratories.
旨在检测人诺如病毒（NoV）的传统广谱逆转录聚合酶链反应（RT-PCR）和实时RT-PCR检测方法也能有效检测猪诺如病毒。对引物和探针结合位点的研究表明，猪诺如病毒基因组序列与人类引物序列之间存在高度同源性。这些发现对食品诊断方法和实验室有严重影响。
