用于检测硬壳蛤（Mercenaria mercenaria）寄生虫QPX（破囊壶菌科）的定量实时聚合酶链反应检测法。
Quantitative real-time PCR assay for QPX (Thraustochytriidae), a parasite of the hard clam (Mercenaria mercenaria).
作者信息
Liu Qianqian, Allam Bassem, Collier Jackie L
机构信息
School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY 11794-5000, USA.
出版信息
Appl Environ Microbiol. 2009 Jul;75(14):4913-8. doi: 10.1128/AEM.00246-09. Epub 2009 May 22.
Abstract
We developed a real-time quantitative PCR (qPCR) assay targeting the rRNA internal transcribed spacer region of the hard clam pathogen QPX. The qPCR assay was more sensitive than was histology in detecting clams with light QPX infections. QPX was detected in 4 of 43 sediment samples but in none of 40 seawater samples.
摘要
我们开发了一种针对硬壳蛤病原体QPX的rRNA内转录间隔区的实时定量PCR（qPCR）检测方法。在检测轻度感染QPX的蛤时，qPCR检测方法比组织学方法更灵敏。在43份沉积物样本中有4份检测到了QPX，但在40份海水样本中均未检测到。
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