龟裂链霉菌原生质体融合株固态发酵和深层发酵生产土霉素

Oxytetracycline production in solid state and submerged fermentation by protoplast fusants of Streptomyces rimosus.

作者信息

Yang S S, Kao C Y

机构信息

Department of Agricultural Chemistry, National Taiwan University, Taipei, Republic of China.

出版信息

Proc Natl Sci Counc Repub China B. 1991 Feb;15(1):20-7.

PMID:1946808

Abstract

Streptomyces rimosus TM-55 was treated with 3% EMS, and 29 auxotrophic mutants (AM-1 - AM-29) were isolated from 5457 colonies at a survival rate between 6.6-66.7%. Three sets of the auxotrophic mutants, AM-3 and AM-27, AM-7 and AM-28, and AM-3 and AM-21, were chosen for protoplast fusion with 50% PEG 1000 for 30 minutes at 25 degrees C, and 25 fusants were isolated (f-1 - f-25). In solid substrate, oxytetracycline production of 20% fusants was higher than that of the wild strain, while in submerged fermentation, it was 44%. Oxytetracycline productions of fusants f-1, f-6, f-11, f-12, f-20 and f-21 were lower than that of the wild strain in solid substrate, but this was reversed in submerged fermentation. On the other hand, OTC production of fusant f-8 was higher than that of the wild strain in solid substrate, and this was reversed in submerged fermentation.

摘要

用3%的甲基磺酸乙酯（EMS）处理龟裂链霉菌TM-55，从5457个菌落中分离出29个营养缺陷型突变体（AM-1至AM-29），存活率在6.6%-66.7%之间。选择三组营养缺陷型突变体，即AM-3和AM-27、AM-7和AM-28以及AM-3和AM-21，在25℃下用50%的聚乙二醇1000进行原生质体融合30分钟，分离出25个融合子（f-1至f-25）。在固体培养基中，20%的融合子土霉素产量高于野生菌株，而在深层发酵中，这一比例为44%。融合子f-1、f-6、f-11、f-12、f-20和f-21在固体培养基中土霉素产量低于野生菌株，但在深层发酵中情况相反。另一方面，融合子f-8在固体培养基中土霉素产量高于野生菌株，而在深层发酵中则相反。