产大环内酯类链霉菌的种间原生质体融合

Interspecific protoplast fusion among macrolide-producing streptomycetes.

作者信息

Ikeda H, Inoue M, Tanaka H, Omura S

出版信息

J Antibiot (Tokyo). 1984 Oct;37(10):1224-30. doi: 10.7164/antibiotics.37.1224.

DOI:10.7164/antibiotics.37.1224

Abstract

From auxotrophic and idiotrophic mutants of Streptomyces fradiae (tylosin producer) and Streptomyces sp. AM 4900 (pikromycin producer) or Streptomyces narbonensis (narbomycin producer), prototrophic fusants were obtained at a low frequency by the protoplast fusion technique. In the cross of S. fradiae 261-27E (mycaminose-idiotroph, ilv) and Streptomyces sp. AM 4900 N3-4, (pikronolide-idiotroph, arg), an unstable prototrophic fusant, strain No. 14, produced a macrolide antibiotic which was not produced by the wild type, parent strains, and the productivity was lost within a few times transfer. It was concluded that the fusant was not a recombinant, but a heterokaryon. On the other hand, relatively stable fusants were obtained from the cross of S. fradiae TBM (mycaminose-idiotroph, met) and S. narbonensis NA12US3 (narbonolide-idiotroph, his, str) at a frequency of 3.2 X 10(-5). One of the prototrophic fusants produced narbomycin, which is believed to be due to a result of interspecific recombination.

摘要

从弗氏链霉菌（泰乐菌素产生菌）、链霉菌属AM 4900（匹克霉素产生菌）或纳博讷链霉菌（纳波霉素产生菌）的营养缺陷型和自养型突变体中，通过原生质体融合技术以低频率获得了原养型融合体。在弗氏链霉菌261-27E（麦糖胺自养型，ilv）与链霉菌属AM 4900 N3-4（匹克诺内酯自养型，arg）的杂交中，一个不稳定的原养型融合体，即14号菌株，产生了一种野生型、亲本菌株均未产生的大环内酯类抗生素，且其生产力在几次传代后丧失。得出的结论是，该融合体不是重组体，而是异核体。另一方面，从弗氏链霉菌TBM（麦糖胺自养型，met）与纳博讷链霉菌NA12US3（纳波内酯自养型，his，str）的杂交中以3.2×10⁻⁵的频率获得了相对稳定的融合体。其中一个原养型融合体产生了纳波霉素，据信这是种间重组的结果。