Lis M, Motomatsu T, Chrétien M
Cancer Res. 1977 Aug;37(8 Pt 1):2612-4.
Fluoride-stimulated adenylate cyclase is demonstrated inisolated tumor cells of transplantable rat pituitary tumor MtT-F4 in vitro. The intracellular cyclic adenosine 3':5'-monophosphate is lowered in the cells incubated in the presence of synthetic somatostatin. Contrary to the findings reported for normal pituitary, however, the immunoreactive growth hormone release does not change when either somatostatin or phosphodiesterase inhibitors are present in the incubation medium. The presence of dibutyryl cyclic adenosine 3':5'-monophosphate (5 mM) in the incubation medium does not change the rate of growth hormone release by isolated tumor cells.
在体外培养的可移植大鼠垂体肿瘤MtT-F4的分离肿瘤细胞中，证实了氟化物刺激的腺苷酸环化酶。在合成生长抑素存在的情况下培养的细胞中，细胞内的环磷腺苷酸水平降低。然而，与正常垂体的报道结果相反，当培养介质中存在生长抑素或磷酸二酯酶抑制剂时，免疫反应性生长激素释放没有变化。培养介质中存在二丁酰环磷腺苷酸（5 mM）不会改变分离的肿瘤细胞释放生长激素的速率。
