Lodowska Jolanta, Wolny Daniel, Jaworska-Kik Marzena, Dzierzewicz Zofia, Weglarz Ludmiła
Department of Biochemistry, Medical University of Silesia, Poland.
Pol J Microbiol. 2009;58(1):21-7.
Bacteria of Desulfovibrio desulfuricans species are Gram-negative, anaerobic rods selectively reducing sulphates and colonizing oxygen-free ecosystems. They are ubiquitous in the natural environment and have been also found to reside in the human digestive tract. They are suggested to be involved in the pathogenesis ofulcerative colitis and Crohn's disease. The D. desulfuricans wild strains were isolated from feces and bioptate of patients suffering from various digestive tract disorders. LPSs were isolated from the wild enteric strains and soil type strain La 2226 of D. desulfuricans and analyzed in terms of their 2-keto-3-deoxyoctulosonic acid (Kdo) component content. The obtained spectrophotometric data indicate that Kdo content is characteristic of each of the investigated strains and it ranges from 0.48% to 2.86% (w/w) of the total LPS mass. Statistically significant interstrain differences of Kdo quantity seem to suggest the differences in the O-antigen content. Comparative analysis of Kdo content in LPSs of D. desulfuricans strains in relation to that of the reference endotoxin from Salmonella spp. allows us to suggest that D. desulfuricans bacteria possess O-antigen polysaccharides composed of diverse number of carbohydrate units.
脱硫脱硫弧菌属细菌为革兰氏阴性厌氧菌，呈杆状，能选择性地还原硫酸盐，并在无氧生态系统中定殖。它们在自然环境中普遍存在，也被发现存在于人类消化道中。有研究表明它们与溃疡性结肠炎和克罗恩病的发病机制有关。脱硫脱硫弧菌野生菌株是从患有各种消化道疾病的患者的粪便和活检组织中分离出来的。从脱硫脱硫弧菌的野生肠道菌株和土壤型菌株La 2226中分离出脂多糖，并对其2-酮-3-脱氧辛酸（Kdo）成分含量进行分析。获得的分光光度数据表明，Kdo含量是每个研究菌株的特征，其占总脂多糖质量的0.48%至2.86%（w/w）。Kdo数量在菌株间的统计学显著差异似乎表明O抗原含量存在差异。对脱硫脱硫弧菌菌株脂多糖中Kdo含量与沙门氏菌属参考内毒素的Kdo含量进行比较分析，使我们认为脱硫脱硫弧菌细菌拥有由不同数量碳水化合物单元组成的O抗原多糖。
