Watanabe H, Hendry J H
Cancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.
Radiat Res. 1991 Nov;128(2):222-4.
A technique has been developed for transplanting whole thyroid follicles into the fat pads of recipient thyroidectomized rats to assess the ability of the follicular cells to proliferate and form colonies, i.e., clusters of new follicles. Of the transplanted follicles, 80-90% formed follicle clusters, indicative of the presence of transplanted follicles not containing colony-forming cells (clonogens) or some reproducible degree of transplantation trauma. The initial number of clonogens per regenerative follicle was calculated from data from split-dose experiments to be 3.3 +/- 1.5, and their sensitivity was characterized by a D0 value of 350 +/- 95 cGy. Three clonogens among about 80 epithelial cells per regenerative follicle and the 10-20% of nonregenerative follicles represent an overall colony-forming efficiency of about 3%. This is similar to the value of 2-3% reported by others using single-cell transplantation techniques.
已开发出一种将整个甲状腺滤泡移植到受体甲状腺切除大鼠脂肪垫中的技术，以评估滤泡细胞增殖并形成集落（即新滤泡簇）的能力。在移植的滤泡中，80 - 90%形成了滤泡簇，这表明存在不含集落形成细胞（克隆原）的移植滤泡，或存在某种可重复程度的移植创伤。根据分次剂量实验数据计算，每个再生滤泡中克隆原的初始数量为3.3±1.5，其敏感性以D0值350±95 cGy表征。每个再生滤泡约80个上皮细胞中的三个克隆原以及10 - 20%的非再生滤泡代表总体集落形成效率约为3%。这与其他人使用单细胞移植技术报告的2 - 3%的值相似。
