组蛋白去乙酰化酶 1、2 和 3 在卵巢癌组织中的表达谱。
Expression profile of histone deacetylases 1, 2 and 3 in ovarian cancer tissues.
机构信息
Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center, Seoul, Korea.
出版信息
J Gynecol Oncol. 2008 Sep;19(3):185-90. doi: 10.3802/jgo.2008.19.3.185. Epub 2008 Sep 30.
OBJECTIVE
To investigate the expression levels of histone deacetylase (HDAC) 1, 2, and 3 in ovarian cancer tissues and normal ovarian tissues.
METHODS
Randomly assigned each of six patients with serous, mucinous and endometrioid ovarian cancer were included. Another six patients with normal ovarian tissue were included for comparison. RT-PCR was performed to quantify the levels of HDACs1-3 mRNA in the cancer and normal tissues. Western blot analysis was performed to measure the expression levels of HDACs1-3 protein. The HDACs1-3 expression pattern was also topologically examined by immunohistochemistry.
RESULTS
Increased mRNA expressions of HDCA1, HDAC 2 and HDAC 3 were detected in 83%, 67% and 83% of 18 cancer tissue samples, compared to normal tissue samples. The relative densities of HDAC1 mRNA and HDAC3 mRNA in the serous, mucinous and endometrioid cancer tissues, and HDAC2 mRNA in serous cancer tissues were significantly higher than those of the normal tissues, respectively (p<0.05). Overexpression of HDAC1, HDAC2 and HDAC3 proteins were detected in 94%, 72% and 83% of 18 cancer samples, respectively. The relative densities of HDAC1 protein and HDAC3 protein in serous, mucinous and endometrioid cancer, and HDAC2 protein in serous and mucinous cancer tissues were significantly higher than those of normal tissues, respectively (p<0.05). Most cancer tissues expressed moderate to strong staining of HDACs1, 2 and 3 in immunohistochemistry. Staining of HDAC2 was weak in only one endometrioid cancer tissue.
CONCLUSION
HDACs1-3 are over expressed in ovarian cancer tissues and probably play a significant role in ovarian carcinogenesis.
目的
研究组蛋白去乙酰化酶（HDAC）1、2 和 3 在卵巢癌组织和正常卵巢组织中的表达水平。
方法
随机选择 6 名浆液性、黏液性和子宫内膜样卵巢癌患者，另选择 6 名正常卵巢组织患者作为对照。采用 RT-PCR 定量分析癌症和正常组织中 HDACs1-3mRNA 的水平，采用 Western blot 分析测量 HDACs1-3 蛋白的表达水平，并用免疫组织化学法拓扑检查 HDACs1-3 的表达模式。
结果
在 18 例癌症组织样本中，HDCA1、HDAC2 和 HDAC3 的 mRNA 表达均增加，分别为 83%、67%和 83%，与正常组织样本相比。在浆液性、黏液性和子宫内膜样卵巢癌组织中 HDAC1mRNA 和 HDAC3mRNA 的相对密度，以及浆液性卵巢癌组织中 HDAC2mRNA 的相对密度均明显高于正常组织（p<0.05）。在 18 例癌症样本中，HDAC1、HDAC2 和 HDAC3 蛋白的过表达分别为 94%、72%和 83%。在浆液性、黏液性和子宫内膜样卵巢癌组织中 HDAC1 蛋白和 HDAC3 蛋白的相对密度，以及浆液性和黏液性卵巢癌组织中 HDAC2 蛋白的相对密度均明显高于正常组织（p<0.05）。在免疫组织化学染色中，大多数癌症组织表达中等至强的 HDACs1、2 和 3 染色。在一个子宫内膜样卵巢癌组织中 HDAC2 的染色较弱。
结论
HDACs1-3 在卵巢癌组织中过度表达，可能在卵巢癌发生中起重要作用。
Zotero 插件