Canada's Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, 675 West 10th Ave., Vancouver, BC, V5Z 1L3, Canada.
Cancer Causes Control. 2009 Oct;20(8):1387-96. doi: 10.1007/s10552-009-9365-4. Epub 2009 May 29.
Human papilloma virus (HPV) prevalence studies performed in different regions and population groups across Canada would inform public health decisions regarding implementation of anti-HPV vaccines.
A total of 8,700 liquid-based specimens from 8,660 women aged 13-86 from throughout British Columbia were collected. DNA was isolated from 4,980 of these samples and assessed for HPV prevalence and type distribution. HPV was detected by PCR analysis using tagged GP5+/6+ consensus primers to amplify the L1 region of HPV; typing was done by bi-directional sequencing of PCR products.
Overall HPV prevalence was 16.8% (age adjusted 15.5%). Prevalence of high-risk HPV was 13.9, and 10.7% of samples contained HPV16. HPV prevalence was highest in the youngest group of women (<20 years). One-third of HPV positive samples contained more than one HPV type. Percentages of low-grade (LGIL) and high-grade intraepithelial lesions (HGIL) containing high-risk HPV are 52.3 and 79.4%, respectively.
Overall HPV prevalence in this study is within the range of estimates from other studies. The prevalence of HPV16 is higher than what is found in other Canadian and international studies. HPV16 and HPV18 compose a majority of the high-risk virus in this study. Use of current HPV vaccines could considerably reduce HPV-related conditions including cervical cancer and procedures such as colposcopy.
在加拿大不同地区和人群中进行的人乳头瘤病毒（HPV）流行率研究，将为有关实施抗 HPV 疫苗的公共卫生决策提供信息。
共采集了来自不列颠哥伦比亚省各地 8660 名年龄在 13-86 岁的 8700 名女性的液体基标本。从其中 4980 个样本中提取 DNA，并评估 HPV 流行率和类型分布。使用标记的 GP5+/6+ 共识引物通过 PCR 分析检测 HPV，以扩增 HPV 的 L1 区；通过 PCR 产物的双向测序进行分型。
总体 HPV 流行率为 16.8%（年龄调整后为 15.5%）。高危型 HPV 的流行率为 13.9%，10.7%的样本中含有 HPV16。HPV 流行率在年龄最小的女性组（<20 岁）中最高。三分之一的 HPV 阳性样本中含有一种以上的 HPV 类型。含有高危型 HPV 的低度（LGIL）和高度上皮内病变（HGIL）的百分比分别为 52.3%和 79.4%。
本研究中的 HPV 总体流行率在其他研究的估计范围内。HPV16 的流行率高于其他加拿大和国际研究中的流行率。HPV16 和 HPV18 构成了本研究中大多数高危病毒。使用当前的 HPV 疫苗可以大大减少 HPV 相关疾病，包括宫颈癌和阴道镜检查等程序。
