Rossi E D, Mulè A, Russo R M, Pierconti F, Fadda G
Division of Anatomic Pathology and Histology, Catholic University, Rome, Italy.
Pathologica. 2008 Dec;100(6):461-5.
Thin-layer cytology (TLC) is an automated method for processing cells harvested in a liquid solution and collected onto a single slide. The leftover material can be used for other techniques such as immunocytochemistry, molecular biology and flow cytometry. TLC has been applied with good results in exfoliative cytology of pulmonary, urinary, gastrointestinal and oral districts as well as in the evaluation of serous effusions. The main advantages of TLC over conventional techniques (CS) are: (a) simplification of the sampling technique; (b) decrease in cellular artefacts leading to a lesser amount of inadequate diagnoses; and (c) applicability of additional investigations. The limits of TLC are: (a) changes in the morphologic picture of some lesions; (b) increase of the workload for technical staff; and (c) increased cost. The application of TLC to non-gynaecologic specimens favours many innovative developments and can be regarded as an appropriate substitute for CS.
薄层细胞学检查（TLC）是一种用于处理在液体溶液中收获并收集到单个载玻片上的细胞的自动化方法。剩余材料可用于其他技术，如免疫细胞化学、分子生物学和流式细胞术。TLC已成功应用于肺、泌尿、胃肠和口腔区域的脱落细胞学检查以及浆液性积液的评估。与传统技术（CS）相比，TLC的主要优点是：（a）简化采样技术；（b）减少细胞假象，从而减少不充分诊断的数量；（c）可进行额外检查。TLC的局限性在于：（a）某些病变形态图像的改变；（b）技术人员工作量增加；（c）成本增加。TLC在非妇科标本中的应用有利于许多创新发展，可被视为CS的合适替代方法。
