Rodrigues Ana Sofia, Lopes Ana Rita, Leão Ana, Couceiro Alda, Ribeiro Ana Bela Sarmento, Ramos Fernando, Noronha da Silveira Maria Irene, Resende de Oliveira Catarina
Grupo de Bromatologia-Centro de Estudos Farmacêuticos, Faculdade de Farmácia da Universidade de Coimbra, 3000-295 Coimbra, Portugal.
J Chromatogr Sci. 2009 May-Jun;47(5):387-91. doi: 10.1093/chromsci/47.5.387.
A high-performance liquid chromatography-UV methodology (lambda=230 nm) was developed and validated for the simultaneous determination of vincristine and doxorubicin in pharmaceutical preparations used in oncology. The chromatography was carried out on a C18 column using acetonitrile 90% in water-potassium hydrogenphosphate buffer 50 mM, pH 3.2+/-0.1 (32:68, v/v) as mobile phase at a flow rate of 1.5 mL/min. The method proved to be specific, exact, and accurate, in accordance with the ICH standards, presenting linearity in the 1-5 microg/mL and 5-100 microg/mL intervals, and detection (0.19x0.51 microg/mL) and quantification (0.63x1.7 microg/mL) limits for vincristine and doxorubicin, respectively.
开发并验证了一种高效液相色谱 - 紫外检测方法（波长λ = 230 nm），用于同时测定肿瘤学用药物制剂中的长春新碱和多柔比星。色谱分析在C18柱上进行，以90%乙腈 - 50 mM磷酸氢二钾缓冲液（pH 3.2 ± 0.1，32:68，v/v）作为流动相，流速为1.5 mL/min。根据国际协调会议（ICH）标准，该方法具有特异性、精密性和准确性，在1 - 5 μg/mL和5 - 100 μg/mL区间呈线性，长春新碱和多柔比星的检测限分别为（0.19×0.51 μg/mL）和定量限分别为（0.63×1.7 μg/mL）。
