Miranda A, Mickle A, Medda B, Zhang Z, Phillips R J, Tipnis N, Powley T L, Shaker R, Sengupta J N
Division of Gastroenterology, Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Neuroscience. 2009 Sep 15;162(4):1299-306. doi: 10.1016/j.neuroscience.2009.05.042. Epub 2009 May 27.
Several types of gastric surgeries have been associated with early satiety, dyspepsia and food intolerances. We aimed to examine alterations in gastric vagal afferents following gastric surgery-fundus ligation.
Six week old, male Sprague-Dawley rats underwent chronic ligation (CL) of the fundus. Sham rats underwent abdominal surgery, but without ligation. Another group of rats underwent acute ligation (AL) of the fundus immediately prior to experiments. CL and sham rats were allowed to grow to age 3-4 months. Food intake and weights were recorded post-operatively. Gastric compliance and gastric wall thickness was measured at baseline and during gastric distension (GD). Extracellular recordings were made to examine response characteristics of vagal afferent fibers to GD and to map the stomach receptive field (RF). The morphological structures of afferent terminals in the stomach were examined with retrograde tracings from the nodose ganglion.
The CL group consumed significantly less food and weighed less than sham control. The mean compliance of the CL group was significantly less than control, but higher than the AL group. The spontaneous firing and responses to GD of afferent fibers from the CL rats were significantly higher than AL rats. There was a marked expansion of the gastric RF in the CL rats with significant reorganization and regeneration of intramuscular array (IMA) terminals. There was no difference in total wall or muscle thickness among the groups.
CL results in aberrant remodeling of IMAs with expansion of the gastric RF and alters the mechanotransduction properties of vagal afferent fibers. These changes could contribute to altered sensitivity following gastric surgery.
几种胃部手术都与早饱、消化不良和食物不耐受有关。我们旨在研究胃部手术——胃底结扎术后胃迷走传入神经的变化。
六周龄雄性Sprague-Dawley大鼠接受胃底慢性结扎（CL）。假手术组大鼠接受腹部手术，但不进行结扎。另一组大鼠在实验前立即接受胃底急性结扎（AL）。CL组和假手术组大鼠饲养至3 - 4月龄。记录术后的食物摄入量和体重。在基线和胃扩张（GD）期间测量胃顺应性和胃壁厚度。进行细胞外记录以检查迷走传入纤维对GD的反应特性，并绘制胃感受野（RF）。通过从结状神经节逆行追踪来检查胃内传入终末的形态结构。
CL组的食物摄入量明显少于假手术对照组，体重也较轻。CL组的平均顺应性明显低于对照组，但高于AL组。CL组大鼠传入纤维的自发放电和对GD的反应明显高于AL组大鼠。CL组大鼠的胃RF有明显扩大，肌内排列（IMA）终末有显著的重组和再生。各组之间的总壁厚度或肌肉厚度没有差异。
CL导致IMA异常重塑，胃RF扩大，并改变迷走传入纤维的机械转导特性。这些变化可能导致胃部手术后敏感性改变。
