Hu Mingqian, Wang Jiongkun, Zhao Hongxia, Dong Shisong, Cai Jiye
Department of Chemistry, Jinan University, Guangzhou, Guangdong 510632, China.
Institution for Tissue Transplantation and Immunology, Jinan University, Guangzhou, Guangdong 510632, China.
J Biomech. 2009 Jul 22;42(10):1513-1519. doi: 10.1016/j.jbiomech.2009.03.051. Epub 2009 May 27.
The ultrastructural and mechanical properties of single resting, activated and apoptosis lymphocyte have been investigated by atomic force microscopy (AFM). Using topographic imaging, we showed that the surface of the resting lymphocyte is smooth, while lymphocyte activation and apoptosis are often accompanied by changes in cell morphology. The apoptosis lymphocyte is rougher than those of the two other morphotypes, and coated with many big particles. Using spatially resolved force-distance curves, we found that the valve of the activated lymphocyte is about two to three times stiffer (Young's modulus of approximately 20 kPa) than those of the two other morphotypes (5-11 kPa). These results can improve our understanding of the mechanical properties of cells during growth and differentiation.
利用原子力显微镜（AFM）研究了单个静息、活化和凋亡淋巴细胞的超微结构及力学特性。通过形貌成像，我们发现静息淋巴细胞的表面是光滑的，而淋巴细胞的活化和凋亡常伴随着细胞形态的变化。凋亡淋巴细胞比其他两种形态类型的淋巴细胞表面更粗糙，且覆盖有许多大颗粒。利用空间分辨力-距离曲线，我们发现活化淋巴细胞的硬度（杨氏模量约为20 kPa）约为其他两种形态类型淋巴细胞（5-11 kPa）的两到三倍。这些结果有助于我们更好地理解细胞在生长和分化过程中的力学特性。
