毛细管电泳法测定红细胞中硫嘌呤甲基转移酶活性

Capillary electrophoresis determination of thiopurine methyl transferase activity in erythrocytes.

作者信息

Tomkova Jana, Friedecky David, Polynkova Adriana, Adam Tomas

机构信息

Laboratory for Inherited Metabolic Disorders, Department of Clinical Biochemistry, University Hospital and Palacky University Olomouc, Olomouc, Czech Republic.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Jul 1;877(20-21):1943-5. doi: 10.1016/j.jchromb.2009.05.005. Epub 2009 May 13.

DOI:10.1016/j.jchromb.2009.05.005

PMID:19477696

Abstract

Thiopurine S-methyltransferase (TPMT) catalyzes methylation of thiopurine drugs (e.g. 6-mercaptopurine, azathioprine). Decreased activity of TPMT is associated with hematopoietic toxicity after administration of standard doses of the drugs. We developed capillary electrophoretic method for determination of TPMT enzyme activity in erythrocytes. Limit of quantification of the method is 1.5 micromol/L (S/N=6). The recovery of 6-methylmercaptopurine was 87.5-94.8%, imprecision value (as CV, n=10) was 1.68% (within-day) and 2.53% (between-day). Erythrocyte TPMT activities were measured in 60 healthy adult volunteers.

摘要

硫嘌呤甲基转移酶（TPMT）催化硫嘌呤类药物（如6-巯基嘌呤、硫唑嘌呤）的甲基化反应。TPMT活性降低与给予标准剂量药物后的造血毒性相关。我们开发了一种毛细管电泳法来测定红细胞中的TPMT酶活性。该方法的定量限为1.5微摩尔/升（信噪比=6）。6-甲基巯基嘌呤的回收率为87.5%-94.8%，不精密度值（以CV表示，n=10）日内为1.68%，日间为2.53%。对60名健康成年志愿者的红细胞TPMT活性进行了测定。