Zimic Mirko, Pajuelo Mónica, Rueda Daniel, López César, Arana Yanina, Castillo Yesenia, Calderón Maritza, Rodriguez Silvia, Sheen Patricia, Vinetz Joseph M, Gonzales Armando, García Héctor H, Gilman Robert H
Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú.
Am J Trop Med Hyg. 2009 Jun;80(6):964-70.
Neurocysticercosis, an endemic parasitic disease in most developing countries, is caused by Taenia solium and compromises the human central nervous system. Cathepsin L-like proteases are secreted by several parasites including T. solium and constitute important antigens for immunodiagnostics. A protein fraction with cathepsin L-like activity was purified from the cysticercus fluid by size exclusion and ion exchange chromatography. Cathepsin L-like activity was measured fluorometrically by detecting the hydrolysis of the fluorogenic substrate Z-Phe-Arg-AMC. The purified protein fraction included antigens of 53 and 25 kD that were tested in a Western immunoblot and in an enzyme-linked immunosorbent assay (ELISA) for detection of human cysticercosis. The sensitivity of the Western immunoblot was 96% for patients infected with multiple cysts and 78% for patients with a single cyst. Specificity was 98%. The sensitivity of the ELISA was 98% in patients with multiple cysts and 84% in patients with a single cyst. Specificity was 92.7%.
神经囊尾蚴病是大多数发展中国家的一种地方性寄生虫病，由猪带绦虫引起，会损害人类中枢神经系统。组织蛋白酶L样蛋白酶由包括猪带绦虫在内的几种寄生虫分泌，是免疫诊断的重要抗原。通过尺寸排阻色谱和离子交换色谱从囊尾蚴液中纯化出具有组织蛋白酶L样活性的蛋白质组分。通过检测荧光底物Z-苯丙氨酸-精氨酸-7-氨基-4-甲基香豆素的水解，用荧光法测定组织蛋白酶L样活性。纯化的蛋白质组分包括53kD和25kD的抗原，在蛋白质免疫印迹法和酶联免疫吸附测定（ELISA）中对其进行检测人类囊尾蚴病的测试。蛋白质免疫印迹法对感染多个囊肿患者的敏感性为96%，对单个囊肿患者的敏感性为78%。特异性为98%。ELISA对多个囊肿患者的敏感性为98%，对单个囊肿患者的敏感性为84%。特异性为92.7%。
