酿酒酵母α-甘露糖苷酶（Cvt途径的货物蛋白）的结晶
Crystallization of Saccharomyces cerevisiae alpha-mannosidase, a cargo protein of the Cvt pathway.
作者信息
Watanabe Yasunori, Noda Nobuo N, Honbou Kazuya, Suzuki Kuninori, Sakai Yasuyoshi, Ohsumi Yoshinori, Inagaki Fuyuhiko
机构信息
Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, N-12, W-6, Kita-ku, Sapporo 060-0812, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jun 1;65(Pt 6):571-3. doi: 10.1107/S1744309109015826. Epub 2009 May 22.
Saccharomyces cerevisiae alpha-mannosidase (Ams1) is a cargo protein that is transported to the vacuole by the cytoplasm-to-vacuole targeting (Cvt) pathway during conditions of growth and by autophagy during conditions of starvation. After transport to the vacuole, Ams1 functions as a resident hydrolase. Ams1 has been overexpressed in the methylotrophic yeast Pichia pastoris, purified and crystallized in two crystal forms. Form I belongs to space group P2(1), with unit-cell parameters a = 145.7, b = 127.7, c = 164.0 A, beta = 101.5 degrees . Form II belongs to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 127.9, b = 163.7, c = 291.5 A. Diffraction data were collected from these crystals to a resolution of 3.3 A for form I and of 2.6 A for form II using synchrotron radiation.
酿酒酵母α-甘露糖苷酶（Ams1）是一种货物蛋白，在生长条件下通过细胞质到液泡靶向（Cvt）途径运输到液泡，在饥饿条件下通过自噬运输到液泡。运输到液泡后，Ams1作为驻留水解酶发挥作用。Ams1已在甲基营养型酵母巴斯德毕赤酵母中过表达，纯化并以两种晶体形式结晶。晶型I属于空间群P2(1)，晶胞参数a = 145.7，b = 127.7，c = 164.0 Å，β = 101.5°。晶型II属于空间群I222或I2(1)2(1)2(1)，晶胞参数a = 127.9，b = 163.7，c = 291.5 Å。使用同步辐射从这些晶体收集衍射数据，晶型I的分辨率为3.3 Å，晶型II的分辨率为2.6 Å。
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