小鼠泪腺腺泡细胞内钙通道的鉴定及功能分布
Identification and functional distribution of intracellular ca channels in mouse lacrimal gland acinar cells.
作者信息
Medina-Ortiz W E, Gregg E V, Brun-Zinkernagel A M, Koulen P
机构信息
Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
出版信息
Open Ophthalmol J. 2007 Dec 4;1:8-16. doi: 10.2174/1874364100701010008.
We have determined the presence and cellular distribution of intracellular calcium channels, inositol 1, 4, 5-trisphosphate receptors (IP3Rs) and ryanodine receptors (RyRs) in adult and postnatal (P10) lacrimal gland acinar cells. Western blot analysis of both P10 cultures and adult tissue identified the presence of each IP(3)R and RyR isotypes. The immunocytochemistry analysis showed a differential cellular distribution of these calcium channels where the nuclear envelope, endoplasmic reticulum (ER) and Golgi apparatus membranes represent areas with highest levels of channel expression. This IP(3)R and RyR isotype distribution is confirmed by the immuno-EM results. The findings described in this study are in agreement with published pharmacological data that shows the participation of these channels in the secretion process of the lacrimal gland acinar cells. Furthermore, the differential subcellular distribution between the isoforms could indicate a potential role of these intracellular Ca(2+ )channels on the regulation of specific cellular functions.
我们已经确定了成年和出生后（P10）泪腺腺泡细胞中细胞内钙通道、肌醇1,4,5-三磷酸受体（IP3Rs）和兰尼碱受体（RyRs）的存在及其细胞分布。对P10培养物和成年组织进行的蛋白质免疫印迹分析确定了每种IP(3)R和RyR亚型的存在。免疫细胞化学分析显示这些钙通道的细胞分布存在差异，其中核膜、内质网（ER）和高尔基体膜是通道表达水平最高的区域。免疫电镜结果证实了这种IP(3)R和RyR亚型分布。本研究中描述的发现与已发表的药理学数据一致，这些数据表明这些通道参与了泪腺腺泡细胞的分泌过程。此外，亚型之间不同亚细胞分布可能表明这些细胞内Ca(2+)通道在特定细胞功能调节中具有潜在作用。
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