Paranyuk Y, Claros N, Birzgalis A, Moore L C, Brink P R, Walcott B
Department of Physiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794-5230, USA.
Curr Eye Res. 2001 Sep;23(3):199-205. doi: 10.1076/ceyr.23.3.199.5468.
The fluid secretory impairment of lacrimal and salivary glands in Sjögren's syndrome (SS) is thought to be related to the extent of lymphocytic infiltration (LI) and subsequent loss of glandular tissue. In this study, we examine the correlation between the extent of tear flow reduction and the extent of LI of lacrimal glands in the NZB/W mouse, a model of SS.
We stimulated tear production by topical application of carbachol onto the gland while fluid was collected from the lacrimal duct. The lacrimal glands were removed after fluid collection for histology.
Fluid secretion in response to carbachol was less in the majority of young NZB/W females compared to C57 control animals and none of the glands showed LI. Fluid secretion was also impaired in the majority of old NZB/W females, and the extent of LI was highly variable. Some of the old SW females also showed blunted fluid secretory responses and some degree of focal LI. Young SW females showed no LI and most animals exhibited normal flow responses. Analysis of paired flow and LI measurements showed no correlation between LI and flow impairment in any of the groups or in the pooled data. Carbachol-stimulated protein secretion from lacrimal gland slices in vitro were similar in young and old SW and NZB/W mice.
These results suggest that LI alone is not sufficient to explain the secretory dysfunction in the NZB/W mouse model of Sjögren's syndrome.
干燥综合征（SS）患者泪腺和唾液腺的液体分泌受损被认为与淋巴细胞浸润（LI）程度及随后的腺组织丧失有关。在本研究中，我们检测了新西兰黑/白（NZB/W）小鼠（一种SS模型）泪液分泌减少程度与泪腺LI程度之间的相关性。
我们通过在泪腺局部应用卡巴胆碱刺激泪液分泌，同时从泪道收集液体。收集液体后取出泪腺进行组织学检查。
与C57对照动物相比，大多数年轻NZB/W雌性小鼠对卡巴胆碱的液体分泌较少，且所有腺体均未显示LI。大多数老年NZB/W雌性小鼠的液体分泌也受损，且LI程度高度可变。一些老年NZB/W雌性小鼠还表现出液体分泌反应迟钝和一定程度的局灶性LI。年轻NZB/W雌性小鼠未显示LI，且大多数动物表现出正常的泪液分泌反应。对配对的泪液分泌和LI测量结果进行分析显示，在任何组或汇总数据中，LI与泪液分泌受损之间均无相关性。年轻和老年NZB/W小鼠体外泪腺切片的卡巴胆碱刺激蛋白分泌相似。
这些结果表明，仅LI不足以解释NZB/W小鼠干燥综合征模型中的分泌功能障碍。
