甘草酸对阿霉素诱导的大鼠肾病综合征的保护作用。

Protective effect of glycyrrhizin on nephrotic syndrome induced by adriamycin in rats.

作者信息

Bi Xuecheng, Zhu Gang, Han Zhaodong, Ye Yongkang, Liang Yuxiang, Zhang Lei, Hao Zhihong, Zeng Guohua, He Huichan, Zhong Weide

机构信息

Guangzhou First Municipal People's Hospital, Affiliated Guangzhou Medical College, Guangzhou 510180, China.

出版信息

Clin Invest Med. 2009 Jun 1;32(3):E229-38.

PMID:19480741

Abstract

PURPOSE

To explore the protective effect of glycyrrhizin in rats with nephrotic syndrome (NS) induced by adriamycin (ADR).

METHODS

36 Sprague Dawley (SD) male rats were divided into control, untreated and glycyrrhizin treatment groups. The NS rat model was established by injecting ADR twice in the untreated and in the glycyrrhizin treatment groups. Rats in the glycyrrhizin treatment group were fed glycyrrhizin by intragastric administration for 7 days. Changes in the following indices were observed in the three groups before and 4 weeks after the treatment: 24 h urine protein quantitation (UPr), serum cholesterol (Ch), serum albumin (Alb), blood urea nitrogen (BUN), serum creatinine (sCr), laminin (LN), fibronectin (FN), collagen (Col), transforming growth factor beta1 (TGFbeta1) and connective tissue growth factor (CTGF); histopathology by light and electron microscope. Expression of LN, FN, ColIV, TGFbeta1 and CTGF in the cortex of the kidney were detected by semi-quantitative immunohistochemical analysis. Expression of TGFbeta1 and CTGF in the cortex of the kidney was detected by Fluorescein Based Quantitive RT-PCR. Macrophage infiltration was evaluated by the immunoperoxidase staining.

RESULTS

Compared with the control group, 24 h UPr, Ch, BUN and sCr of rats in the untreated group were increased. Glycyrrhizin reduced 24 h Upr, Ch, BUN, sCr, LN, FN, Col, TGFbeta1, CTGF, and mean arterial blood pressure. Pathological changes in the kidney, the expression of LN, FN, Col, TGFbeta1 and CTGF in the cortex of the kidney in the glycyrrhizin treatment group were decreased compared with the untreated group. Glycyrrhizin also suppressed macrophage infiltration in the kidneys of NS rat models.

CONCLUSION

Glycyrrhizin exerts protective effects in rats with NS, reducing the excretion of Upr, Ch, BUN, sCr, and mean arterial blood pressure, and also decreasing expression of LN, FN, Col, TGFbeta1 and CTGF in the kidney. Renal function is improved and the severity of NS is lessened.

摘要

目的

探讨甘草酸对阿霉素（ADR）诱导的肾病综合征（NS）大鼠的保护作用。

方法

将36只雄性Sprague Dawley（SD）大鼠分为对照组、未治疗组和甘草酸治疗组。未治疗组和甘草酸治疗组通过两次注射ADR建立NS大鼠模型。甘草酸治疗组大鼠通过灌胃给予甘草酸7天。观察三组治疗前及治疗4周后以下指标的变化：24小时尿蛋白定量（UPr）、血清胆固醇（Ch）、血清白蛋白（Alb）、血尿素氮（BUN）、血清肌酐（sCr）、层粘连蛋白（LN）、纤连蛋白（FN）、胶原蛋白（Col）、转化生长因子β1（TGFβ1）和结缔组织生长因子（CTGF）；光镜和电镜下的组织病理学。通过半定量免疫组织化学分析检测肾皮质中LN、FN、ColIV、TGFβ1和CTGF的表达。通过基于荧光的定量RT-PCR检测肾皮质中TGFβ1和CTGF的表达。通过免疫过氧化物酶染色评估巨噬细胞浸润。

结果

与对照组相比，未治疗组大鼠的24小时UPr、Ch、BUN和sCr升高。甘草酸降低了24小时Upr、Ch、BUN、sCr、LN、FN、Col、TGFβ1、CTGF和平均动脉血压。与未治疗组相比，甘草酸治疗组肾脏的病理变化、肾皮质中LN、FN、Col、TGFβ1和CTGF的表达降低。甘草酸还抑制了NS大鼠模型肾脏中的巨噬细胞浸润。