Shiota Seiji, Mannen Kazuaki, Matsumoto Takashi, Yamada Kentaro, Yasui Takehito, Takayama Katsuyoshi, Kobayashi Yukuharu, Khawplod Pakamatz, Gotoh Kazuyo, Ahmed Kamruddin, Iha Hidekatsu, Nishizono Akira
Department of Microbiology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu-City, Oita, Japan.
J Virol Methods. 2009 Oct;161(1):58-62. doi: 10.1016/j.jviromet.2009.05.018. Epub 2009 May 27.
The level of virus-neutralizing antibody, which plays a crucial role in the prevention of rabies, is determined by rabies virus (RABV) neutralizing test, which are time- and cost-consuming. In order to determine the level of neutralizing antibody in vaccinees, an easy and reliable method is needed. Based on the principle of immunochromatography, we developed a RAPINA (RAPId Neutralizing Antibody) test to determine the presence of neutralizing antibody in serum. In the RAPINA test, if neutralizing antibody equivalent to 0.5IU/ml of serum sample are mixed with an optimal amount of inactivated RABV (iRABV) and are completely absorbed by the virus, none of the iRABV can bind with monoclonal antibody that recognizes the iRABV glycoprotein (G) on the test strip. A total of 115 human sera samples were tested. The sensitivity, specificity and accuracy of the RAPINA test compared with rapid fluorescent focus inhibition test (RFFIT) as a standard test, were 88.7, 91.9 and 90.4%, respectively. The RAPINA test is a simple, safe and rapid method, which can be a substitute for neutralizing tests that use live viruses, cultured cells and fluorescence microscopy. This test might be useful for screening a large number of sera.
病毒中和抗体水平在狂犬病预防中起着关键作用，其通过狂犬病病毒（RABV）中和试验来测定，该试验耗时且成本高。为了确定疫苗接种者体内的中和抗体水平，需要一种简便可靠的方法。基于免疫层析原理，我们开发了一种RAPINA（快速中和抗体）试验来检测血清中中和抗体的存在。在RAPINA试验中，如果血清样本中相当于0.5IU/ml的中和抗体与适量的灭活RABV（iRABV）混合，并被病毒完全吸收，那么iRABV就无法与检测条上识别iRABV糖蛋白（G）的单克隆抗体结合。共检测了115份人血清样本。与作为标准试验的快速荧光灶抑制试验（RFFIT）相比，RAPINA试验的敏感性、特异性和准确性分别为88.7%、91.9%和90.4%。RAPINA试验是一种简单、安全且快速的方法，可替代使用活病毒、培养细胞和荧光显微镜的中和试验。该试验可能有助于大量血清的筛查。
Zotero 插件