Wang S H, Zheng H J
Guizhou Provincial Institute of Parasitic Diseases, Guiyang, People's Republic of China.
Southeast Asian J Trop Med Public Health. 1991 Jun;22(2):165-7.
Methods were studied for the cryopreservation of microfilariae of periodic Brugia malayi. RPMI-1640 tissue culture medium containing 6% dimethyl sulfoxide (DMSO) and 15% newborn calf serum was used as cryoprotectant. Samples were frozen slowly in the vapor phase of liquid nitrogen prior to emersion in liquid nitrogen (-196 degrees C). The freezing rate was -0.5 to -1.0 degrees C per minute, microfilariae remained viable for as long as, 212 and 375 days, survival rates were 94 to 98% and they were infective to Aedes togoi mosquitos. The infective larvae (L3) were obtained for 10-11 days after feeding at 28 degrees C room-temperature and the infection rate of L3 in test mosquitos was 22.4-30.6%. All DMSO should be removed from the freezing medium to restore microfilariae activity after freezing.
研究了周期性马来布鲁线虫微丝蚴的冷冻保存方法。含有6%二甲基亚砜（DMSO）和15%新生牛血清的RPMI-1640组织培养基用作冷冻保护剂。样品在浸入液氮（-196℃）之前，先在液氮的气相中缓慢冷冻。冷冻速率为每分钟-0.5至-1.0℃，微丝蚴可存活长达212天和375天，存活率为94%至98%，并且它们对东乡伊蚊具有感染性。在28℃室温下喂食10 - 11天后可获得感染性幼虫（L3），试验蚊虫中L3的感染率为22.4% - 30.6%。冷冻后应从冷冻培养基中去除所有DMSO以恢复微丝蚴活性。
