Wu Yicong, Xi Peng, Qu Jianan, Cheung Tak-Hong, Yu Mei-Yung
Opt Express. 2004 Jul 12;12(14):3218-23. doi: 10.1364/opex.12.003218.
A confocal fluorescence spectroscopy system is instrumented to study depth-resolved autofluorescence in biological tissue. The system provides the capability of optical sectioning with the maximal detectiondepth up to 120 m in the examined tissue samples. It was found that the topmost keratinizing epithelial layer produces strong fluorescence similar to collagen. The fluorescence signal from epithelial tissue between the keratinizing layer and stroma can be well resolved. The study results show that depth-resolved fluorescence spectroscopy has the potential to provide more accurate information for the diagnosis of tissue pathology.
共聚焦荧光光谱系统被用于研究生物组织中深度分辨的自体荧光。该系统具备光学切片能力，在所检测的组织样本中最大检测深度可达120微米。研究发现，最顶层的角质化上皮层产生与胶原蛋白相似的强荧光。角质化层与基质之间的上皮组织的荧光信号能够被很好地分辨出来。研究结果表明，深度分辨荧光光谱有潜力为组织病理学诊断提供更准确的信息。
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