Tagami Yuichi, Kurimoto Takuji, Miyoshi Tomomitsu, Morimoto Takeshi, Sawai Hajime, Mimura Osamu
Department of Ophthalmology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
Jpn J Ophthalmol. 2009 May;53(3):257-66. doi: 10.1007/s10384-009-0657-8. Epub 2009 May 31.
To investigate whether electrical stimulation promoted axonal regeneration of retinal ganglion cells (RGCs) after optic nerve (ON) crush in adult rats.
Transcorneal electrical stimulation (TES), which stimulates the retina with current from a corneal contact lens electrode, was used to stimulate the eye. TES was applied for 1 h immediately after ON crush. Axonal regeneration was determined by anterograde labeling of RGC axons. To examine whether the axonal regeneration was mediated by insulin-like growth factor 1 (IGF-1) receptors, an IGF-1 receptor antagonist, JB3, was injected intraperitoneally before each TES application. Immunostaining for IGF-1 was performed to examine the effects of TES. To test the survival-promoting effects of TES applied daily, the mean density of retrogradely labeled RGCs was determined on day 12 after ON crush.
Compared with sham stimulation, the mean number of regenerating axons significantly increased at 250 microm distal from the lesion and increased IGF-1 immunoreactivity was observed in retinas treated daily with TES. Preinjection of an IGF-1 receptor antagonist significantly blocked axonal regeneration by TES applied daily. TES applied daily also markedly enhanced the survival of RGCs 12 days after ON crush.
TES applied daily promotes both axonal regeneration and survival of RGCs after ON crush.
研究电刺激是否能促进成年大鼠视神经挤压伤后视网膜神经节细胞（RGCs）的轴突再生。
采用经角膜电刺激（TES），即通过角膜接触镜电极施加电流刺激视网膜。在视神经挤压伤后立即进行1小时的TES。通过RGC轴突的顺行标记来确定轴突再生情况。为了研究轴突再生是否由胰岛素样生长因子1（IGF-1）受体介导，在每次TES施加前腹腔注射IGF-1受体拮抗剂JB3。进行IGF-1免疫染色以检测TES的效果。为了测试每日进行TES的促存活作用，在视神经挤压伤后第12天测定逆行标记的RGCs的平均密度。
与假刺激相比，损伤部位远端250微米处再生轴突的平均数量显著增加，并且在每日接受TES治疗的视网膜中观察到IGF-1免疫反应性增加。预先注射IGF-1受体拮抗剂可显著阻断每日进行的TES所诱导的轴突再生。每日进行TES还显著提高了视神经挤压伤后12天RGCs的存活率。
每日进行TES可促进视神经挤压伤后RGCs的轴突再生和存活。
