Singh B P, Behera B K
Industrial Biotechnology Laboratory, Advanced Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, India.
Lett Appl Microbiol. 2009 Aug;49(2):254-9. doi: 10.1111/j.1472-765X.2009.02652.x. Epub 2009 May 27.
In the present communication, attempts have been made to regulate the tacrolimus production by supplementing commercial source of carbons and amino acids timely.
Tacrolimus production was regulated by supplying vegetable oils and amino acids, individually and in combination. Tacrolimus quantification was done by HPLC. Streptomyces spp. MA6858 B3178 was found to produce 115.3 mg l(-1) of tacrolimus. The rotation speed of shake flask, pH of the broth and supply of air were maintained at 7.1, 230 rev min(-1) and 2.0 vv m air respectively.
The effect of carbons on tacrolimus production was noticed to be of diphasic manner. During the first 24 h of culture, monosaccharide is used for the growth of microbe. However, after the lapse of 36 h, addition of soya oil and l-lysine in combination enhanced the tacrolimus production to 115.3 mg l(-1). Besides this, pH of broth was also noticed as a critical factor in monitoring tacrolimus biosynthesis.
The newly isolated mutant Streptomyces spp. MA6858 B3178 having high potential for tacrolimus production as compared to existing data can be well used for the commercialization of tacrolimus.
在本通讯中，已尝试通过及时补充商业来源的碳源和氨基酸来调节他克莫司的产量。
通过单独或联合供应植物油和氨基酸来调节他克莫司的产量。他克莫司的定量分析采用高效液相色谱法。发现链霉菌属MA6858 B3178可产生115.3 mg l(-1)的他克莫司。摇瓶的转速、培养液的pH值和空气供应量分别维持在7.1、230转/分钟和2.0 vv m空气。
注意到碳源对他克莫司产量的影响呈双相方式。在培养的最初24小时内，单糖用于微生物的生长。然而，在36小时后，联合添加大豆油和L-赖氨酸可使他克莫司产量提高到115.3 mg l(-1)。除此之外，培养液的pH值也是监测他克莫司生物合成的关键因素。
与现有数据相比，新分离的具有高他克莫司生产潜力的链霉菌属突变体MA6858 B3178可很好地用于他克莫司的商业化生产。
