Stroud Jacqueline L, Paton Graeme I, Semple Kirk T
Department of Environmental Science, The Lancaster Environment Centre, Lancaster University, UK.
Chemosphere. 2009 Sep;77(1):123-8. doi: 10.1016/j.chemosphere.2009.05.005. Epub 2009 May 31.
Slurry-based mineralisation assays are widely used to investigate contaminant biodegradation in soil; however, the importance of shaking speed on microbial degradation has not been considered. This study investigated the mineralisation of (14)C-analogues of phenanthrene, hexadecane and octacosane, shaken at 0, 25 and 100 rpm. The results showed that the fastest rates and highest levels of mineralisation in 0 d aged soils were in the highly agitated conditions (100 rpm). However, the highest levels of mineralisation in 500 d aged soil were found in the gently shaken conditions (25 rpm), with the levels of mineralisation significantly (p<0.05) one third higher than under the highly agitated conditions (100 rpm). Consequently, estimation of the maximum levels of biodegradation of organic contaminants in aged soil systems should be considered under gentle mixing conditions.
基于泥浆的矿化分析被广泛用于研究土壤中污染物的生物降解；然而，尚未考虑振荡速度对微生物降解的重要性。本研究调查了菲、十六烷和二十八烷的（14）C类似物在0、25和100转/分钟振荡条件下的矿化情况。结果表明，在0天龄土壤中，矿化速率最快和水平最高的是在高度搅拌条件下（100转/分钟）。然而，在500天龄土壤中，矿化水平最高的是在轻轻振荡条件下（25转/分钟），矿化水平比高度搅拌条件下（100转/分钟）显著高出三分之一（p<0.05）。因此，在老化土壤系统中估计有机污染物生物降解的最大水平时应考虑温和混合条件。
