Torres Rogelio R, Arias Maria C, Moretto Geraldo
Depto. de Producion Animal, Univ. Nacional de Colombia, Bogotá D.C.
Neotrop Entomol. 2009 Mar-Apr;38(2):208-12. doi: 10.1590/s1519-566x2009000200007.
The geographical distribution of the Brazilian endemic stingless bee Melipona quadrifasciata quadrifasciata Lepeletier ranges from Rio Grande do Sul to Minas Gerais states. The objective of the present study was to verify mtDNA polymorphisms among samples of M. q. quadrifasciata collected in southern Brazil. Twenty nine colonies from three localities (Blumenau and Mafra/SC and Prudentópolis/ PR) were sampled. Seven mtDNA regions were amplified and further digested with 15 restriction enzymes (PCR-RFLP). Five composite haplotypes were identified, with two unique to samples from Prudentópolis and the remaining three to samples from Mafra and/or Blumenau.
巴西本土无刺蜂四带无刺蜂梅利波纳亚种（Melipona quadrifasciata quadrifasciata Lepeletier）的地理分布范围从南里奥格兰德州到米纳斯吉拉斯州。本研究的目的是验证在巴西南部采集的四带无刺蜂样本中的线粒体DNA多态性。从三个地点（布卢梅瑙和马夫拉/圣卡塔琳娜州以及普鲁登托波利斯/巴拉那州）采集了29个蜂群样本。扩增了七个线粒体DNA区域，并用15种限制性内切酶进行进一步消化（聚合酶链反应-限制性片段长度多态性分析，PCR-RFLP）。鉴定出五种复合单倍型，其中两种是普鲁登托波利斯样本所特有的，其余三种是马夫拉和/或布卢梅瑙样本所特有的。
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