基于点阵寡核苷酸微阵列的比较基因组杂交技术。
Comparative genomic hybridization on spotted oligonucleotide microarrays.
作者信息
Kim Young H, Pollack Jonathan R
机构信息
Department of Pathology, Stanford University, Stanford, CA, USA.
出版信息
Methods Mol Biol. 2009;556:21-32. doi: 10.1007/978-1-60327-192-9_3.
Recent advances in DNA microarray technology have enabled researchers to comprehensively characterize the complex genomes of higher eukaryotic organisms at an unprecedented level of detail. Array-based comparative genomic hybridization (Array-CGH) has been widely used for detecting DNA copy number alterations on a genomic scale, where the mapping resolution is limited only by the number of probes on the DNA microarray. In this chapter, we present a validated protocol utilizing print-tip spotted HEEBO (Human Exonic Evidence Based Oligonucleotide) microarrays for conducting array-CGH using as little as 25 ng of genomic DNA from a wide variety of sources, including cultured cell lines and clinical specimens, with high spatial resolution and array-to-array reproducibility.
DNA微阵列技术的最新进展使研究人员能够以前所未有的详细程度全面表征高等真核生物的复杂基因组。基于阵列的比较基因组杂交（Array-CGH）已被广泛用于在基因组规模上检测DNA拷贝数变化，其定位分辨率仅受DNA微阵列上探针数量的限制。在本章中，我们介绍了一种经过验证的方案，该方案利用打印点样的HEEBO（基于人类外显子证据的寡核苷酸）微阵列，使用低至25 ng来自多种来源（包括培养的细胞系和临床标本）的基因组DNA进行Array-CGH，具有高空间分辨率和阵列间的可重复性。
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