Li Ji, Jiang Yunyun, Wen Jun, Fan Guorong, Wu Yutian, Zhang Chuan
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, People's Republic of China.
Biomed Chromatogr. 2009 Nov;23(11):1201-7. doi: 10.1002/bmc.1244.
This paper describes a sensitive, specific and rapid high-performance liquid chromatography (HPLC) method for the determination of curcumin in rat plasma. After a simple step of protein precipitation in 96-well format using acetonitrile containing the internal standard (IS), emodin, plasma samples were analyzed by reverse-phase HPLC. Curcumin and the IS emodin were separated on a Diamonsil C(18) analytical column (4.6 x 100 mm, 5 microm) using acetonitrile-5% acetic acid (75:25, v/v) as mobile phase at a flow rate of 1.0 mL/min. The method was sensitive with a lower limit of quantitation of 1 ng/mL, with good linearity (r(2) >or= 0.999) over the linear range 1-500 ng/mL. All the validation data, such as accuracy and precision, were within the required limits. A run time of 3.0 min for each sample made high-throughput bioanalysis possible. The assay method was successfully applied to the study of the pharmacokinetics of curcumin liposome in rats.
本文描述了一种灵敏、特异且快速的高效液相色谱（HPLC）法，用于测定大鼠血浆中的姜黄素。在96孔板中使用含内标（IS）大黄素的乙腈进行简单的蛋白沉淀步骤后，通过反相HPLC分析血浆样品。姜黄素和内标大黄素在Diamonsil C(18)分析柱（4.6×100 mm，5μm）上分离，以乙腈-5%乙酸（75:25，v/v）为流动相，流速为1.0 mL/min。该方法灵敏，定量下限为1 ng/mL，在1-500 ng/mL的线性范围内具有良好的线性（r(2)≥0.999）。所有验证数据，如准确度和精密度，均在规定范围内。每个样品的运行时间为3.0分钟，使得高通量生物分析成为可能。该测定方法成功应用于姜黄素脂质体在大鼠体内的药代动力学研究。
