Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education (MAHE), 576104, Manipal, Karnataka, India.
School of Pharmacy, Faculty of Health Sciences, University of Botswana, Gaborone, Botswana.
J Fluoresc. 2022 Jul;32(4):1517-1527. doi: 10.1007/s10895-022-02947-w. Epub 2022 May 8.
Curcumin (CUR), a natural polyphenolic compound extracted from the rhizomes of Curcuma longa, is used as a pharmaceutical agent, spice in food, and as a dye. Currently, CUR is being investigated for cancer treatment in Phase-II clinical trials. CUR also possesses excellent activities like anti-inflammatory, anti-microbial, and anti-oxidant, therefore quality control is crucial. The present research work was to develop a new, simple, validated and time-saving rapid 96-well plate spectrofluorimetric method for the determination of CUR. The developed method was compared with routinely used high performance liquid chromatography (HPLC) technique. The developed method were found to be linear in the concentration range of 15 to 3900 ng/mL with R ≥ 0.9983 for spectrofluorimetric and 50-7500 ng/mL with R ≥ 0.9999 for HPLC method. Accuracy, intraday and interday precision was adequate, with RSD lower than the suggested limits. The limits for the detection and the quantification of CUR were 7 and 15 ng/mL for spectrofluorimetric, and 25 and 50 ng/mL for HPLC respectively. The Bland-Altman analysis demonstrated the similarities between the two methods. The 96-well plate method was successfully applied to determine CUR in solid lipid nanoparticles (SLNs) and chitosan nanoparticles (Chi-NPs). The developed spectrofluorimetric method can hence serve as a possible replacement for the HPLC method for the quantification of CUR in healthcare and food products.
姜黄素（CUR）是从姜黄根茎中提取的天然多酚化合物，用作药物制剂、食品香料和染料。目前，CUR 正在进行 II 期临床试验以用于癌症治疗。CUR 还具有出色的抗炎、抗菌和抗氧化活性，因此质量控制至关重要。本研究旨在开发一种新的、简单的、经过验证的、节省时间的快速 96 孔板荧光分光光度法来测定 CUR。所开发的方法与常规使用的高效液相色谱（HPLC）技术进行了比较。所开发的方法在 15 至 3900ng/mL 的浓度范围内具有线性关系，荧光分光光度法的 R≥0.9983，HPLC 法的 R≥0.9999。准确度、日内和日间精密度均足够，RSD 低于建议的限度。CUR 的检测限和定量限分别为荧光分光光度法的 7 和 15ng/mL，HPLC 法的 25 和 50ng/mL。Bland-Altman 分析表明两种方法具有相似性。96 孔板法成功应用于测定固体脂质纳米粒（SLNs）和壳聚糖纳米粒（Chi-NPs）中的 CUR。因此，所开发的荧光分光光度法可替代 HPLC 法用于保健品和食品中 CUR 的定量分析。
