Ujvari Beata, Madsen Thomas
School of Biological Sciences, University of Wollongong, Wollongong, NSW, Australia.
Mitochondrial DNA. 2008 Oct;19(5):465-70. doi: 10.1080/19401730802449162.
Using PCR, the complete mitochondrial genome was sequenced in three frillneck lizards (Chlamydosaurus kingii). The mitochondria spanned over 16,761bp. As in other vertebrates, two rRNA genes, 22 tRNA genes and 13 protein coding genes were identified. However, similar to some other squamate reptiles, two control regions (CRI and CRII) were identified, spanning 801 and 812 bp, respectively. Our results were compared with another Australian member of the family Agamidae, the bearded dragon (Pogana vitticeps). The overall base composition of the light-strand sequence largely mirrored that observed in P vitticeps. Furthermore, similar to P. vitticeps, we observed an insertion 801 bp long between the ND5 and ND6 genes. However, in contrast to P vitticeps we did not observe a conserved sequence block III region. Based on a comparison among the three frillneck lizards, we also present data on the proportion of variable sites within the major mitochondrial regions.
利用聚合酶链式反应（PCR），对三只褶伞蜥（鬃狮蜥）的线粒体基因组进行了全序列测定。线粒体全长超过16,761碱基对。与其他脊椎动物一样，鉴定出了两个核糖体RNA基因、22个转运RNA基因和13个蛋白质编码基因。然而，与其他一些有鳞目爬行动物类似，鉴定出了两个控制区（CRI和CRII），分别跨越801和812碱基对。我们的结果与鬣蜥科的另一个澳大利亚成员鬃狮蜥（鬃狮蜥）进行了比较。轻链序列的总体碱基组成在很大程度上反映了在鬃狮蜥中观察到的情况。此外，与鬃狮蜥类似，我们在ND5和ND6基因之间观察到一个801碱基对长的插入片段。然而，与鬃狮蜥不同的是，我们没有观察到保守序列块III区域。基于对三只褶伞蜥的比较，我们还给出了主要线粒体区域内可变位点比例的数据。
