[牙周病原菌群体感应信号系统luxS基因的检测]
[Detection of luxS gene of quorum sensing signal system in periodontal pathogen].
作者信息
Lei Chao-Feng, Yang He, Sun Chang-Juan, Miao Di, Xu Yi
机构信息
Department of Periodontology, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2009 Jan;44(1):32-4.
OBJECTIVE
To detect the presence and distribution of luxS gene in quorum sensing signal system in the periodontal pathogens.
METHODS
The total DNA of Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), Actinobacillus acitinomycetimcomtans (Aa) were extracted. The presence of luxS was detected by polymerase chain reaction (PCR). The products of PCR were detected by electrophoresis, sequenced and identified by a Blast search of the GenBank database.
RESULTS
Electrophoresis, sequencing and Blast searching indicated that the PCR products of Pg were highly consistent with the luxS gene in GenBank. The sequencing result of Fn was also identified with the target gene. The PCR product of Aa was the same as reference through electrophoresis.
CONCLUSIONS
Pg, Fn, Aa contain luxS gene. Further studies may be required to investigate the functions of luxS in the periodontal pathogens.
目的
检测牙周病原体群体感应信号系统中luxS基因的存在及分布情况。
方法
提取牙龈卟啉单胞菌（Pg）、具核梭杆菌（Fn）、放线共生放线杆菌（Aa）的总DNA。采用聚合酶链反应（PCR）检测luxS的存在情况。PCR产物经电泳检测、测序，并通过GenBank数据库的Blast搜索进行鉴定。
结果
电泳、测序及Blast搜索表明，Pg的PCR产物与GenBank中的luxS基因高度一致。Fn的测序结果也与目标基因相符。Aa的PCR产物经电泳与参照一致。
结论
Pg、Fn、Aa含有luxS基因。可能需要进一步研究以探讨luxS在牙周病原体中的功能。
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