Hudson Andrew J, Floate Kevin D
University of Lethbridge, 4401 University Drive, Lethbridge, Alberta T1K 3M4, Canada.
J Parasitol. 2009 Dec;95(6):1545-7. doi: 10.1645/GE-2145.1.
We used molecular techniques to characterize bacteria associated with the nematomorph Gordius robustus (Leidy). This worm is a parasite of the fall field cricket, Gryllus pennsylvanicus (Burmeister), which is infected with the symbiotic bacteria, Wolbachia. Because of this close association, our a priori expectation was that G. robustus may be similarly infected. However, results of denaturing gradient gel electrophoresis and sequencing of amplified 16S rDNA failed to detect any bacteria (symbiotic or non-symbiotic) in G. robustus. These unexpected findings suggest that G. robustus has no internal bacterial community and indicate that close association with a Wolbachia-infected host is insufficient for the transmission of bacteria from insect to nematomorph.
我们运用分子技术对与强壮铁线虫（Leidy）相关的细菌进行了特征描述。这种线虫是宾夕法尼亚蟋蟀（Burmeister）的寄生虫，而宾夕法尼亚蟋蟀感染了共生细菌沃尔巴克氏体。由于这种紧密关联，我们先验地预期强壮铁线虫可能也受到类似感染。然而，变性梯度凝胶电泳结果以及扩增的16S rDNA测序未能在强壮铁线虫中检测到任何细菌（共生或非共生）。这些意外发现表明强壮铁线虫没有内部细菌群落，并表明与感染沃尔巴克氏体的宿主紧密关联并不足以使细菌从昆虫传播到线虫。
