Mearini Ettore, Antognelli Cinzia, Del Buono Chiara, Cochetti Giovanni, Giannantoni Antonella, Nardelli Emanuela, Talesa Vincenzo N
University of Perugia, Department of Medical-Surgical Specialties and Public Health, Division of Urology and Andrology, Perugia, Italy.
Biomarkers. 2009 Jun;14(4):235-43. doi: 10.1080/13547500902807306.
The identification of reliable molecular biomarkers in prostate cancer early diagnosis is clinically desirable. We quantitatively detected prostate cancer specifically overexpressed genes, DD3 and PSA, in urine sediments of men suffering from prostate cancer or benign prostate hyperplasia, after prostatic massage. As both genes are exclusively expressed in androgen receptor expressing human prostate carcinoma cell lines, we further investigated the possible effect of androgens on PSA and DD3 gene expression. DD3 and PSA mRNA levels were measured by real-time polymerase chain reaction. The combined markers test had a sensitivity of 80.2% and a specificity of 100%. Both gene transcripts were significantly upregulated by androgens. Results indicated the clinical usefulness of the combination of DD3 and PSA as molecular markers in the early diagnosis of prostate cancer and the need of combining as many as possible analytical data with the clinical and demographic ones to achieve the maximum level of diagnostic accuracy.
在前列腺癌早期诊断中识别可靠的分子生物标志物具有临床需求。我们在前列腺按摩后，对患有前列腺癌或良性前列腺增生的男性尿液沉淀物中前列腺癌特异性过表达基因DD3和PSA进行了定量检测。由于这两个基因仅在表达雄激素受体的人前列腺癌细胞系中表达，我们进一步研究了雄激素对PSA和DD3基因表达的可能影响。通过实时聚合酶链反应测量DD3和PSA mRNA水平。联合标志物检测的灵敏度为80.2%，特异性为100%。两种基因转录本均被雄激素显著上调。结果表明，DD3和PSA联合作为分子标志物在前列腺癌早期诊断中具有临床实用性，并且需要将尽可能多的分析数据与临床和人口统计学数据相结合，以实现最大程度的诊断准确性。
