Weiss Louis M, Fiser Andras, Angeletti Ruth Hogue, Kim Kami
Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Forchheimer 504, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Expert Rev Proteomics. 2009 Jun;6(3):303-13. doi: 10.1586/epr.09.16.
Toxoplasma gondii is a ubiquitous, Apicomplexan parasite that, in humans, can cause several clinical syndromes, including encephalitis, chorioretinitis and congenital infection. T. gondii was described a little over 100 years ago in the tissues of the gundi (Ctenodoactylus gundi). There are a large number of applicable experimental techniques available for this pathogen and it has become a model organism for the study of intracellular pathogens. With the completion of the genomes for a type I (GT-1), type II (ME49) and type III (VEG) strains, proteomic studies on this organism have been greatly facilitated. Several subcellular proteomic studies have been completed on this pathogen. These studies have helped elucidate specialized invasion organelles and their composition, as well as proteins associated with the cytoskeleton. Global proteomic studies are leading to improved strategies for genome annotation in this organism and an improved understanding of protein regulation in this pathogen. Web-based resources, such as EPIC-DB and ToxoDB, provide proteomic data and support for studies on T. gondii. This review will summarize the current status of proteomic research on T. gondii.
刚地弓形虫是一种广泛存在的顶复门寄生虫，在人类中可引发多种临床综合征，包括脑炎、脉络膜视网膜炎和先天性感染。刚地弓形虫于100多年前在非洲梳趾鼠（栉趾鼠）的组织中被发现。针对这种病原体有大量适用的实验技术，它已成为研究细胞内病原体的模式生物。随着I型（GT-1）、II型（ME49）和III型（VEG）菌株基因组测序的完成，对该生物体的蛋白质组学研究得到了极大推动。已经完成了几项针对这种病原体的亚细胞蛋白质组学研究。这些研究有助于阐明专门的入侵细胞器及其组成，以及与细胞骨架相关的蛋白质。整体蛋白质组学研究正在为该生物体的基因组注释带来改进策略，并增进对该病原体蛋白质调控的理解。基于网络的资源，如EPIC-DB和ToxoDB，为刚地弓形虫研究提供蛋白质组学数据及支持。本综述将总结刚地弓形虫蛋白质组学研究的现状。
