Kaji M, Mikawa H
Department of Pediatrics, Kyoto University School of Medicine, Japan.
Toxicology. 1991;69(2):143-9. doi: 10.1016/0300-483x(91)90226-q.
Intraperitoneal injection of valproic acid (VPA) induces metallothionein (MT) in mouse liver. There was a clear-cut dose-dependency and the maximal amount of MT induced by VPA was about 6 times the basal level. The hepatic MT level reached the peak value at 24 h after VPA injection. Neither Cu nor Zn concentrations showed any significant changes after VPA administration in either the whole liver, or in the mitochondrial or supernatant fractions of the liver homogenate. Gel filtration profiles for the supernatant of the liver homogenate of the VPA-treated mice, however, clearly showed that induced MT was chiefly Zn-thionein. Therefore, Zn molecules necessary for the elevated MT levels seem to originate mainly from cytosolic Zn-containing proteins except for MT.
腹腔注射丙戊酸（VPA）可诱导小鼠肝脏中的金属硫蛋白（MT）。存在明确的剂量依赖性，VPA诱导的MT最大量约为基础水平的6倍。VPA注射后24小时肝脏MT水平达到峰值。给予VPA后，全肝、肝匀浆的线粒体或上清液部分的铜和锌浓度均未显示出任何显著变化。然而，VPA处理小鼠肝脏匀浆上清液的凝胶过滤图谱清楚地表明，诱导的MT主要是锌硫蛋白。因此，MT水平升高所需的锌分子似乎主要来源于除MT之外的含锌胞质蛋白。
