Cosimi Simona, Orta Luis, Mateos Santiago, Cortés Felipe
Department of Cell Biology, Faculty of Biology, University of Seville, Av. Reina Mercedes 6, E-41012 Seville, Spain.
Toxicol In Vitro. 2009 Sep;23(6):1110-5. doi: 10.1016/j.tiv.2009.05.017. Epub 2009 May 31.
Ochratoxin A (OTA), a known nephrotoxin and carcinogenic mycotoxin, was investigated to examine its effectiveness to induce cytotoxicity and DNA damage (Comet assay), as well as its possible inhibition of topoisomerase II (topo II) catalytic activity in cultured Chinese hamster ovary (CHO) cells. The analysis of OTA-induced DNA strand breaks as well as the flow cytometric assessment of polyploidy has provided evidence that is consistent with the idea of a mixed mode of action of the mycotoxin: in addition to its genotoxic activity, OTA may also interfere with chromosome distribution during cell division.
赭曲霉毒素A（OTA）是一种已知的肾毒素和致癌霉菌毒素，本研究旨在考察其诱导细胞毒性和DNA损伤（彗星试验）的效果，以及其对培养的中国仓鼠卵巢（CHO）细胞中拓扑异构酶II（topo II）催化活性的可能抑制作用。对OTA诱导的DNA链断裂的分析以及多倍体的流式细胞术评估提供了证据，这些证据与霉菌毒素混合作用模式的观点一致：除了其遗传毒性活性外，OTA还可能在细胞分裂过程中干扰染色体分布。
